Monitoring the activity of the mitotic kinase PLK-1 (Polo-Like Kinase 1) in vivo using a FRET-based biosensor in the early C. elegans embryo

Diplôme Master Monitoring the activity of the mitotic kinase PLK-1 (Polo-Like Kinase 1) in vivo using a FRET-based biosensor in the early C. elegans embryo eng 2014 author Jesus François 777617 director Gotta Monica 0000-0002-4336-7860 659510 The asymmetric cell division is a process by which a cell divides in two daughter cells with different fates. Asymmetric cell division is fundamental in animals. In particular for generating various cell types and also for preserving the stem cell pool. During asymmetric cell division, the cell needs to coordinate cell cycle events with cell polarity. Indeed, the polarization of the cell permits the segregation of numerous cell determinants which is essential for the specialization of the cell. The coupling of polarization and cell cycle is finely regulated. However, the mechanisms of the coupling are still not fully understood. The nematode Caenorhabditis elegans is particularly well suited for the study of the links between cell cycle and polarity. The first five cleavages of the zygote are asymmetric and the proteins in action are well conserved across species. Among the actors cross-talking between cell cycle and cell polarity, the mitotic kinase PLK-1 (Polo-Like Kinase 1) plays a role. PLK-1 regulates both cell cycle and cell polarity. The aim of this work is to elaborate tools allowing us to monitor the activity of PLK-1. To achieve this, we focused on using FRET-based biosensors and antibodies. The FRET-based biosensors were developed to follow the PLK-1 spatiotemporal activity in vivo. In addition, an antibody against active form (phosphorylated) of PLK-1 was produced and its specificity was investigated. PLK-1 Cell cycle Cell polarity C. elegans FRET Dewey 590 Dewey 610 Master en génétique, développement et évolution (GDE) 44 Département de génétique et évolution 162 49 Section de médecine fondamentale 21 false ddab1d43-d6c5-4609-8d96-4896f77d0672 Master thesis Private F Jesus archives pdf.pdf 10155175 application/pdf closedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution Faculté de médecine / Section de médecine fondamentale

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Monitoring the activity of the mitotic kinase PLK-1 (Polo-Like Kinase 1) in vivo using a FRET-based biosensor in the early C. elegans embryo				2014	298	2

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Jesus, François