Etude phytochimique de Lannea acida et son activité antiproliférative sur les cellules souches cancéreuses du myélome multiple

Diplôme Master Etude phytochimique de Lannea acida et son activité antiproliférative sur les cellules souches cancéreuses du myélome multiple eng 2017 author Bruna Laure 773059 director Cuendet Licea Muriel 0000-0002-1523-1907 686755 Muriel Cuendet Lannea acida A. Rich. (Anacardiaceae) is a west African plant used in traditional medicine. In a course of an activity screening in Multiple Myeloma Cancer Stem Cells, the dichloromethane extract of the plant's root bark inhibited the cell growth at 20 μg/mL (<30% of cell growth). A bioassay-guided fractionation was performed to isolate and identify active molecules that inhibits the cell proliferation, using analytical techniques such as High Performance Liquid Chromatography, Mass Spectrometry, Nuclear Magnetic Resonance, Infrared, Ultraviolet-visible and Circular Dichroism spectroscopies, Specific rotation and MTT-proliferation assay. Using these techniques, four new and active molecules with similar structures were isolated. They have comparable inhibition concentrations (IC50), surrounding 10-15 μM, in MM-CSCs which are drug resistant cells, responsible of some cancer relapses. Lannea acida A. Rich. Anacardiaceae Bioassay-fractionation Multiple Myeloma Multiple Myeloma Cancer Stem Cells Proliferation assay Dewey 615 Master en Pharmacie 31 Section des sciences pharmaceutiques 17 false 884 Pharmacognosie d1f4a08e-71e9-4f1c-b4df-043f647181cd Master thesis Restricted 170616121402_brunala0__TPR.pdf 8236404 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Etude phytochimique de Lannea acida et son activité antiproliférative sur les cellules souches cancéreuses du myélome multiple				2017	287	10

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