Immunization <i>in vitro</i> and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins

Article Article scientifique Article Immunization <i>in vitro</i> and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins eng 1984 author Van Ness Jeffrey 77082 author Laemmli Ulrich Karl 25441 author Pettijohn David E. 7897-7901 10.1073/pnas.81.24.7897 0027-8424 http://www.pnas.org/cgi/doi/10.1073/pnas.81.24.7897 A procedure is described for immunizing in vitro and stimulating proliferation of specific B-cell lymphocytes. The method is applicable to production of monoclonal antibodies against proteins that are soluble only in denaturing solvents. An induction period is described in which antigen is presented to the B-cell population in the absence of serum. Also, antigen is coupled to mitogenic silica, which allows the effective presentation of both soluble and insoluble antigens. The results indicate hybridomas can be obtained that secrete IgMs directed against highly conserved or weakly immunogenic antigens. Hybridoma Mitogen Antigen presentation Chromosomal protein Immune suppression Dewey 540 Dewey 570 39 Département de biochimie 144 43 Département de biologie moléculaire 161 false Proceedings of the National Academy of Sciences 81 24 54031d13-ff07-48a4-8c6e-041540d0a318 Article (Published version) Public 7897.full.pdf 1212203 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Immunization in vitro and production of monoclonal antibodies specific to insoluble and weakly immunogenic proteins	Proceedings of the National Academy of Sciences			1984	287	188

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Van Ness, Jeffrey