Evolthon: A community endeavor to evolve lab evolution

Article Article scientifique Article Evolthon: A community endeavor to evolve lab evolution eng 2019-03-29 2019-03-29 author Kaminski Strauss Sivan 0000-0002-4220-4592 author Schirman Dvir 0000-0001-5175-0176 author Jona Ghil 0000-0002-4526-6182 author Brooks Aaron N. 0000-0002-5309-7307 author Kunjapur Aditya M. 0000-0001-6869-9530 author Nguyen Ba Alex N. 0000-0003-1357-6386 author Flint Alice 0000-0002-9912-119X author Solt Andras 0000-0003-4035-8421 author Mershin Andreas author Dixit Atray author Yona Avihu H. author Csörgő Bálint author Busby Bede Phillip 0000-0003-3410-9998 author Hennig Bianca P. 0000-0002-7733-8160 author Pál Csaba author Schraivogel Daniel 0000-0002-5734-6980 author Schultz Daniel author Wernick David G. author Agashe Deepa 0000-0002-0374-8159 author Levi Dikla author Zabezhinsky Dmitry author Russ Dor 0000-0003-4372-7958 author Sass Ehud 0000-0003-4302-1496 author Tamar Einat author Herz Elad author Levy Emmanuel 417450 Département de biologie moléculaire et cellulaire (MOCEL) author Church George M. author Yelin Idan 0000-0003-2515-4113 author Nachman Iftach 0000-0002-4667-0398 author Gerst Jeffrey E. 0000-0002-8411-6881 author Georgeson Joseph M. author Adamala Katarzyna P. 0000-0003-1066-7207 author Steinmetz Lars M. 0000-0002-3962-2865 author Rübsam Marc author Ralser Markus 0000-0001-9535-7413 author Klutstein Michael 0000-0002-4041-3112 author Desai Michael M. author Walunjkar Nilima author Yin Ning 770749 author Aharon Hefetz Noa author Jakimo Noah author Snitser Olga 0000-0001-6067-1912 author Adini Omri author Kumar Prashant author Soo Hoo Smith Rachel author Zeidan Razi 0000-0002-6012-1563 author Hazan Ronen 0000-0001-9967-730X author Rak Roni 0000-0002-3843-3659 author Kishony Roy author Johnson Shannon 0000-0002-6758-224X author Nouriel Shira author Vonesch Sibylle C. 0000-0003-2485-1048 author Foster Simmie author Dagan Tal 0000-0002-9042-192X author Wein Tanita author Karydis Thrasyvoulos 0000-0001-7148-9665 author Wannier Timothy M. 0000-0002-4165-4751 author Stiles Timothy 0000-0001-7069-2426 author Olin-Sandoval Viridiana 0000-0001-8049-807X author Mueller William F. author Bar-On Yinon M. 0000-0001-8477-609X author Dahan Orna author Pilpel Yitzhak 0000-0003-3200-9344 e3000182 10.1371/journal.pbio.3000182 30925180 1544-9173 PMC6440615 https://dx.plos.org/10.1371/journal.pbio.3000182 In experimental evolution, scientists evolve organisms in the lab, typically by challenging them to new environmental conditions. How best to evolve a desired trait? Should the challenge be applied abruptly, gradually, periodically, sporadically? Should one apply chemical mutagenesis, and do strains with high innate mutation rate evolve faster? What are ideal population sizes of evolving populations? There are endless strategies, beyond those that can be exposed by individual labs. We therefore arranged a community challenge, Evolthon, in which students and scientists from different labs were asked to evolve Escherichia coli or Saccharomyces cerevisiae for an abiotic stress—low temperature. About 30 participants from around the world explored diverse environmental and genetic regimes of evolution. After a period of evolution in each lab, all strains of each species were competed with one another. In yeast, the most successful strategies were those that used mating, underscoring the importance of sex in evolution. In bacteria, the fittest strain used a strategy based on exploration of different mutation rates. Different strategies displayed variable levels of performance and stability across additional challenges and conditions. This study therefore uncovers principles of effective experimental evolutionary regimens and might prove useful also for biotechnological developments of new strains and for understanding natural strategies in evolutionary arms races between species. Evolthon constitutes a model for community-based scientific exploration that encourages creativity and cooperation. NIGMS NIH HHS R01 GM104239 Medical Research Council 1509279 The Francis Crick Institute 10134 true PLoS biology 17 3 2442a6b0-2310-4c7b-8b78-68bd595be98e Article (Published version) Public Article-scientifique-from-unpaywall.pdf 2059452 application/pdf CC BY-4.0 7c79168096b682dfd882f52d57d9c3368e9921ba537df755ead25efb6adefb00 openAccess

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Evolthon: A community endeavor to evolve lab evolution	PLoS biology			2019	75	42

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