The transcription factor Sfp1 regulates growth and division in the yeast Saccharomyces cerevisiae

Diplôme Thèse The transcription factor Sfp1 regulates growth and division in the yeast Saccharomyces cerevisiae eng 2016-07-18 author Tomassetti Susanna 768539 director Shore David M. 102587 10.13097/archive-ouverte/unige:86279 urn:nbn:ch:unige-862792 Sc. 4960 The Split Finger Protein 1 (Sfp1) is an yeast nutrient- and stress-sensitive transcription factor that controls for the expression of growth related genes involved in ribosome biogenesis, rRNA processing and translation. Sfp1 was early recognized as an unusual transcription factor that occupies the promoter of a reduced set of its target genes and its mechanism of action have been remained elusive. Sfp1 ChIP-seq experiments performed in this thesis revealed that Sfp1 may play a direct role in the regulation of RNAPII recruitment during PIC formation and this control does not necessarily require Sfp1 promoter occupancy. We have found that SFP1 positively affects the expression of growth related genes whereas it has a negative effect on stress-sensitive genes expression. Finally, we have described that Sfp1 occupies some cell cycle-dependent gene in a cell cycle dependent manner, suggesting a key role of this transcription factor in the intricate process that maintains cell size homeostasis. Yeast Cell-cycle Cell growth RNA Polymerases Dewey 570 43 Département de biologie moléculaire 161 false e788b020-135f-4f46-8436-3758aba6a445 Thesis Public PhD Thesis Susanna Tomassetti 3-8-2016.pdf 6812087 application/pdf Restricted 2017-08-09 eb2b0e4d47da9adb31c0eaa1572c97e9c549ade0191ea3848631b03284e9c75e openAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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The transcription factor Sfp1 regulates growth and division in the yeast Saccharomyces cerevisiae				2016	1,067	741

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Tomassetti, Susanna