Interphase Nuclear Matrix and Metaphase Scaffolding Structures

Article Article scientifique Article Interphase Nuclear Matrix and Metaphase Scaffolding Structures eng 1984 author Lewis Catherine 73065 author Lebkowski Jane Stéphanie 73064 author Daly Ann Katherine 76837 author Laemmli Ulrich Karl 25441 103-122 10.1242/jcs.1984.Supplement_1.8 0021-9533 http://jcs.biologists.org/cgi/doi/10.1242/jcs.1984.Supplement_1.8 The protein compositions of purified metaphase chromosomes, nuclei and their residual scaffold and matrix structures, are reported. The protein pattern of nuclei on sodium dodecyl sulphate/polyacrylamide gels is considerably more complex and rich in non-histone proteins than that of chromosomes. Nuclei contain about three to four times more non-histone proteins relative to their histones than chromosomes. Besides the protein components of the peripheral lamina, several protein bands are specific or at least highly enriched in nuclei. Conversely, two proteins X0 (33×10³ Mr) and X1 (37×10³ Mr) are highly enriched in the pattern of metaphase chromosomes. We have compared morphologically the previously defined nuclear matrices type I and II. The type I nuclear matrix is composed of the known lamina proteins, which form the peripheral lamina structure, and a complex series of proteins that form the internal network of the matrix as observed by electron microscopy. This internal network is stabilized similarly to the metaphase scaffolding by metalloprotein interaction. Both the scaffolding and the internal network of the matrix dissociate if thiols or certain metal chelators are used in the extraction buffer. Under these conditions the resulting nuclear structure, called matrix type II, appears empty in the electron microscope, with the exception of some residual nucleolar material. This latter material can be extracted from the internal network by exhaustive treatment of the nuclei with RNase before extraction with high salt. Immunoblotting and activity studies show RNA polymerase II to be tightly bound to the type I, but not to the type II matrix, or to the scaffolding structure. No polymerase II enzyme was detected in isolated metaphase chromosomes. Another nuclear enzyme, poly(ADP-ribose) polymerase is not bound to either of the residual nuclear matrices or to the scaffolding structures. The association of RNA polymerase with the internal network of the nuclear matrix is consistent with the idea that transcription occurs in close association with this structure. Dewey 540 Dewey 570 39 Département de biochimie 144 43 Département de biologie moléculaire 161 false Journal of Cell Science 1984 Supplement 1 Higher Order Structure in the Nucleus 7c7006c5-709f-4a11-8f21-fe3564551b81 Article (Published version) Public 103.full.pdf 4597838 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de biologie / Département de biologie moléculaire

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Interphase Nuclear Matrix and Metaphase Scaffolding Structures	Journal of Cell Science			1984	315	157

Archive ouverte UNIGE

Daly, Ann Katherine