Distribution of free and conjugated cannabinoids in human bile samples

Article Article scientifique Article Distribution of free and conjugated cannabinoids in human bile samples eng 2012 author Fabritius Marie 765138 author Staub Christian 59634 author Mangin Patrice 662095 author Giroud Christian 766492 114-118 10.1016/j.forsciint.2012.08.013 22980143 0379-0738 The metabolism of Δ(9)-tetrahydrocannabinol (THC) is relatively complex, and over 80 metabolites have been identified. However, much less is known about the formation and fate of cannabinoid conjugates. Bile excretion is known to be an important route for the elimination of phase II metabolites. A liquid chromatography-tandem mass spectrometry LC-MS/MS procedure for measuring cannabinoids in oral fluid was adapted, validated and applied to 10 bile samples. THC, 11-hydroxy-Δ(9)-tetrahydrocannabinol (11-OH-THC), 11-nor-9-carboxy-Δ(9)-tetrahydrocannabinol (THCCOOH), cannabinol (CBN), cannabidiol (CBD), Δ(9)-tetrahydrocannabinolic acid A (THC-A), 11-nor-9-carboxy-Δ(9)-tetrahydrocannabinol glucuronide (THCCOOH-gluc) and Δ(9)-tetrahydrocannabinol glucuronide (THC-gluc) were determined following solid-phase extraction and LC-MS/MS. High concentrations of THCCOOH-gluc were found in bile samples (range: 139-21,275 ng/mL). Relatively high levels of THCCOOH (7.7-1548 ng/mL) and THC-gluc (38-1366 ng/mL) were also measured. THC-A, the plant precursor of THC, was the only cannabinoid that was not detected. These results show that biliary excretion is an important route of elimination for cannabinoids conjugates and that their enterohepatic recirculation is a significant factor to consider when analyzing blood elimination profiles of cannabinoids. Furthermore, we suggest that the bile is the matrix of choice for the screening of phase II cannabinoid metabolites. Dewey 614.1 67 Institut de médecine légale 22A2 false 522 Groupe Mangin Patrice (médecine légale) 862 Forensic science international 223 1-3 991a1d38-f1b1-45da-8e9d-fef95a691857 Article (Published version) Restricted 2012_Fabritius_DistributionFreeConjugated_ForensScienJour.pdf 174349 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de santé et médecine communautaires / Institut de médecine légale

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Distribution of free and conjugated cannabinoids in human bile samples	Forensic science international			2012	572	0

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Fabritius, Marie