New high throughput screening method for drug release measurements

Article Article scientifique Article New high throughput screening method for drug release measurements eng 2013 author Pelczarska Aleksandra Paulina 760359 author Delie Salmon Florence 121330 author Domańska Urszula author Carrupt Pierre-Alain 135654 author Martel Sophie 135666 151-157 10.1016/j.ejpb.2013.02.012 23958326 0939-6411 In the field of drug delivery systems, microparticles made of polymeric matrix appear as an attractive approach. The in vitro release kinetic profile is crucial information when developing new particulate formulations. These data are essential for batch to batch comparison, quality control as well as for anticipation of in vivo behavior to select the best formulation to go further in preclinical investigations. The methods available present common drawbacks such as the time- and compound-consumption that does not fit with formulation screening requirements in early development stages. In this study, a new microscale high throughput screening (HTS) method has been developed to investigate drug release kinetic from piroxicam-loaded polylactic acid (PLA) and polylactic-co-glycolic acid (PLGA) microparticles. The method is a sample- and separation-based method where separation is performed by filtration using 96-well micro filter plates. 96 experiments can therefore be performed on one plate in one time in a fully automated way and with a very low sample and particle consumption. The influence of different parameters controlling release profiles was also investigated using this technique. The HTS method gave the same release profile than the standard dialysis method. Shaking, particle concentration, and the nature of the release medium were found to be of influence. The HTS method appears as a reliable method to evaluate drug release from particles with smaller standard deviation and less consumption of material. Anti-Inflammatory Agents, Non-Steroidal/administration & dosage/chemistry Automation, Laboratory Chemical Phenomena Delayed-Action Preparations/administration & dosage/chemistry Drug Compounding Filtration High-Throughput Screening Assays Kinetics Lactic Acid/chemistry Microchemistry Microscopy, Electron, Scanning Microspheres Particle Size Piroxicam/administration & dosage/chemistry Polyesters/chemistry Polyglycolic Acid/chemistry Quality Control Reproducibility of Results Solubility Surface Properties Dewey 615 31 Section des sciences pharmaceutiques 17 false 883 Technologie pharmaceutique European Journal of Pharmaceutics and Biopharmaceutics 85 1 526f1df7-b3eb-461a-bc78-59c7ab4b39e0 Article (Published version) Restricted Pelczarska_EJBP_2013.pdf 1198226 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

Title	Published in	Access level	OA Policy	Year	Views	Downloads

New high throughput screening method for drug release measurements	European Journal of Pharmaceutics and Biopharmaceutics			2013	436	0

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Pelczarska, Aleksandra Paulina