Blood virosphere in febrile Tanzanian children

Article Article scientifique Article Blood virosphere in febrile Tanzanian children eng 2021 author Cordey Samuel 507357 author Laubscher Florian 748134 author Hartley Mary-Anne author Junier Thomas author Keitel Kristina author Docquier Mylene 513819 author Guex Nicolas author Iseli Christian 759843 author Vieille Gaël 869505 author Le Mercier Philippe 514147 author Gleizes Anne 651869 author Samaka Josephine author Mlaganile Tarsis author Kagoro Frank author Masimba John author Said Zamzam author Temba Hosiana author Elbanna Gasser H author Tapparel Caroline 0000-0002-0411-6567 27039 author Zanella Marie-Céline 538298 author Xenarios Ioannis 767135 author Fellay Jacques 938391 author D'Acremont Valérie author Kaiser Laurent 28229 982-993 10.1080/22221751.2021.1925161 33929935 2222-1751 Viral infections are the leading cause of childhood acute febrile illnesses motivating consultation in sub-Saharan Africa. The majority of causal viruses are never identified in low-resource clinical settings as such testing is either not part of routine screening or available diagnostic tools have limited ability to detect new/unexpected viral variants. An in-depth exploration of the blood virome is therefore necessary to clarify the potential viral origin of fever in children. Metagenomic next-generation sequencing is a powerful tool for such broad investigations, allowing the detection of RNA and DNA viral genomes. Here, we describe the blood virome of 816 febrile children (<5 years) presenting at outpatient departments in Dar es Salaam over one-year. We show that half of the patients (394/816) had at least one detected virus recognized as causes of human infection/disease (13.8% enteroviruses (enterovirus A, B, C, and rhinovirus A and C), 12% rotaviruses, 11% human herpesvirus type 6). Additionally, we report the detection of a large number of viruses (related to arthropod, vertebrate or mammalian viral species) not yet known to cause human infection/disease, highlighting those who should be on the radar, deserve specific attention in the febrile paediatric population and, more broadly, for surveillance of emerging pathogens.Trial registration: ClinicalTrials.gov identifier: NCT02225769. Dewey 540 Dewey 590 Dewey 616 Swiss National Science Foundation DNA and RNA viruses of the blood virome of allogeneic hematopoietic stem cell transplant recipients: a longitudinal observational study during a one-year period after transplantation - the Geneva Blood Virome Project (the GeBVir Project) 320030_179507 CH Project funding 23 Institut de génétique et de génomique 0DH 39 Département de biochimie 144 44 Département de génétique et évolution 162 54 Département de microbiologie et médecine moléculaire 21B 56 Département de médecine 221 false 400 Virologie clinique 668 931 Identification des facteurs clés impliqués dans la pathogénèse des rhinovirus et entérovirus 953 https://github.com/SAFIA-HTS/VIRAL-HTS Emerging Microbes & Infections 10 1 41f6a324-1562-4c16-a4ee-38c45e5cab23 Article (Published version) Public SCordey_Blood_virosphere_in_febrile_Tanzanian_children.pdf 1584499 application/pdf CC BY fb2faf4d-0fc3-440b-b802-2e36d9b9a60c Supplemental data Public 14694950.zip 5479111 application/zip CC BY openAccess Centres et instituts / Institut de génétique et de génomique Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté des sciences / Section de biologie / Département de génétique et évolution Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire Faculté de médecine / Section de médecine clinique / Département de médecine

Title	Published in	Year	Views	Downloads

Blood virosphere in febrile Tanzanian children	Emerging Microbes & Infections	2021	441	266
Virosaurus A Reference to Explore and Capture Virus Genetic Diversity	Viruses	2020	267	175

Archive ouverte UNIGE

Iseli, Christian