Comparative Analysis of RNA-seq transcriptomes in aging and non-aging Hydra

Diplôme Master Comparative Analysis of RNA-seq transcriptomes in aging and non-aging Hydra eng 2016 author Cuozzo Alessandro 755975 director Galliot Brigitte 0000-0001-7596-8284 29979 The cnidarian Hydra exhibits extremely low senescence when maintained in homeostatic conditions. However H. oligactis animals that undergo sexual differentiation upon transfer from 18°C to 10°C, exhibit a visible aging. Our laboratory characterized two H. oligactis male strains that exhibit different phenotypes once they enter gametogenesis: the cold-sensitive strain (Ho_CS) that exhibits a typical cold-induced degenerative phenotype, and the cold-resistant strain (Ho_CR) that returns to homeostasis. In this study, we performed a large-scale comparative transcriptomic analysis to identify structural as well as regulatory genetic differences between these two strains. We identified a TNF receptor-associated factor (TRAF) that shows a series of deletions in Ho_CS when compared to Ho_CR. We also characterized genes differentially expressed over time between the two strains, such as CIRBP, IMPDH1, TRPA1 and TRPM3, which are orthologs to human proteins involved in response to cold or sensory perception of temperature stimulus, as well as sequences that encode uncharacterized proteins. Moreover, we characterized genes reported in the Human Ageing Genomic Resources (HAGR) GenAge database revealed supplementary interesting candidates such as orthologs for AIFM1, EIF5A2, ERCC4, GCLC, GSK3A, HDAC, HSPD1, MLH1, MAPK3, SOCS2, TRAP1, UCHL1 and ZMPSTE24, which are temporarily differentially expressed between the two strains. TRAP1 and HSPD1 are heat shock proteins, the latter being also involved in the response to cold. We thus retrieved interesting genes candidates that may influence aging phenotype following exposure to cold in Hydra. Transcriptomics RNA-seq Hydra Aging Cold Dewey 590 Maîtrise Universitaire (master) en Biologie, orientation Bioinformatique et Analyse des Données en Biologie 44 Département de génétique et évolution 162 false b6b0fbf8-b2e6-439c-ab39-eaf96ca22286 Master thesis Restricted 2016_Master_Thesis_Alessandro_Cuozzo.pdf 12181386 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution

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Comparative Analysis of RNA-seq transcriptomes in aging and non-aging Hydra				2016	200	12

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Cuozzo, Alessandro