The <i>repA2</i> Gene of the Plasmid R100.1 Encodes a Repressor of Plasmid Replication

Article Article scientifique Article The <i>repA2</i> Gene of the Plasmid R100.1 Encodes a Repressor of Plasmid Replication eng 1983 author Liu Chengpin 75519 author Churchward Gordon author Caro Lucien 25084 148-155 10.1016/0147-619X(83)90067-7 0147-619X https://linkinghub.elsevier.com/retrieve/pii/0147619X83900677 We have constructed two miniplasmids, derived from the resistance plasmid R100.1. In one of these plasmids 400 bp of R100.1 DNA have been replaced by DNA from the transposon Tn1000 (gamma-delta). This substitution removes the amino-terminal end of the repA2 coding sequence of R100.1 and results in an increased copy number of the plasmid carrying the substitution. The copy number of the substituted plasmid is reduced to normal levels in the presence of R100.1. The repA2 gene thus encodes a trans-acting repressor function involved in the control of plasmid replication. Dewey 570 Swiss National Science Foundation 3.169.0.81 CH 43 Département de biologie moléculaire 161 false Plasmid 10 2 08c93793-96b2-422a-886b-b28da10ac870 Article (Published version) Restricted 1-s2.0-0147619X83900677-main.pdf 1086773 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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The repA2 Gene of the Plasmid R100.1 Encodes a Repressor of Plasmid Replication	Plasmid			1983	199	0

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Liu, Chengpin