Highly Potent Cationic Chitosan Derivatives Coupled to Antimicrobial Peptide Dendrimers to Combat Pseudomonas aeruginosa

Article Article scientifique Article Highly Potent Cationic Chitosan Derivatives Coupled to Antimicrobial Peptide Dendrimers to Combat Pseudomonas aeruginosa eng 2024-03-11 2024-07 author Jordan Olivier 0000-0001-6724-5780 Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland.,Section of Pharmaceutical Sciences, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland. 133859 Section des sciences pharmaceutiques (FARMA) author Gan Bee Ha 0000-0001-5899-1837 Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, Bern, 3012, Switzerland. author Alwan Sari Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland.,Section of Pharmaceutical Sciences, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland. 753777 author Perron Karl 0000-0002-3259-4638 Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland.,Section of Pharmaceutical Sciences, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland.,Microbiology Unit, Department of Botany and Plant Biology, University of Geneva, 30 Quai Ernest-Ansermet, Geneva, 1211, Switzerland. 45679 Département des sciences végétales (BIVEG) author Sublet Emmanuelle 0000-0002-1839-2028 Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland.,Section of Pharmaceutical Sciences, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland. 514013 Section des sciences pharmaceutiques (FARMA) author Ducret Verena 0000-0002-2244-3799 Microbiology Unit, Department of Botany and Plant Biology, University of Geneva, 30 Quai Ernest-Ansermet, Geneva, 1211, Switzerland. 596806 Département des sciences végétales (BIVEG) author Ye Hua Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, Oxford, UK. author Borchard Gerrit 0000-0001-6268-2586 Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland.,Section of Pharmaceutical Sciences, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland. 635281 Section des sciences pharmaceutiques (FARMA) author Reymond Jean-Louis 0000-0003-2724-2942 Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, Bern, 3012, Switzerland. 742414 author Patrulea Viorica 0000-0003-3906-2041 Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland.,Section of Pharmaceutical Sciences, University of Geneva, 1 Rue Michel Servet, Geneva, 1211, Switzerland.,Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, OX3 7DQ, Oxford, UK. 813295 Section des sciences pharmaceutiques (FARMA) 2304118 10.1002/adhm.202304118 38412457 2192-2640 https://advanced.onlinelibrary.wiley.com/doi/10.1002/adhm.202304118 The burden of bacterial wound infections has considerably increased due to antibiotic resistance to most of the currently available antimicrobial drugs. Herein, for the first time, a chemical coupling of two cationic N-aryl (pyridyl and aminocinnamyl) chitosan derivatives to antimicrobial peptide dendrimers (AMPDs) of different generations (first, second, and third) via thioether-haloacetyl reaction is reported. The new chitosan-AMPD conjugates show high selectivity by killing Pseudomonas aeruginosa and very low toxicity toward mammalian cells, as well as extremely low hemolysis to red blood cells. Electron microscopy reveals that the new chitosan derivatives coupled to AMPD destroy both the inner and outer membranes of Gram-negative P. aeruginosa. Moreover, chitosan-AMPD conjugates show synergetic effects within extremely low concentrations. The new chitosan-AMPD conjugates can be used as potent antimicrobial therapeutic agents, to eradicate pathogens such as those present in acute and chronic infected wounds. Gram‐negative P. aeruginosa Antimicrobial agents Antimicrobial peptide dendrimers (AMPDs) Antimicrobial peptides (AMPs) Chitosan derivatives Dewey 615 Dewey 580 31 Section des sciences pharmaceutiques 17 45 Département des sciences végétales 163 false 879 Biopharmacie Advanced healthcare materials 13 19 448fb8cf-b5d6-4253-ab6f-5c9260971cb7 Article (Published version) Public Jordan_2024_Highly_Potent_Cationic_.pdf 5460294 application/pdf CC BY-4.0 fe1e1701b68767dc032fc462d782f206782ec36e72be85b1548d7f9b275a6d2d openAccess Faculté des sciences / Section des sciences pharmaceutiques Faculté des sciences / Section de biologie / Département des sciences végétales

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Highly Potent Cationic Chitosan Derivatives Coupled to Antimicrobial Peptide Dendrimers to Combat Pseudomonas aeruginosa	Advanced healthcare materials			2024	0	0

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