Microbiota of healthy dental pulps under the omics loupe

Article Article scientifique Article Microbiota of healthy dental pulps under the omics loupe eng 2025-03-31 2025-03-31 author Berard Alan 750860 CUMD - Clinique de cariologie et d'endodontie (CCAR) author Mauffrey Florian 913522 author Gaia Nadia 800637 author Perez Alexandre 633750 CUMD - Enseignement de chirurgie orale et implantologie (ECOIM) author Chaabane Chiraz 668795 Département de médecine (DEMED) author Schrenzel Jacques 29184 Département de médecine (DEMED) author Leprince Julian Grégoire 405476 CUMD - Division de cariologie et d'endodontie - communs (DCAR) author Bouillaguet Serge 23861 Décanat et administration de la faculté de médecine (ADMED) author Lazarevic Vladimir 0000-0002-6839-2536 684594 Département de médecine (DEMED) 3232 10.3390/ijms26073232 40244028 1422-0067 PMC11989987 https://www.mdpi.com/1422-0067/26/7/3232 This study aims to contribute to the understanding of the dental pulp microbiome through metataxonomic analysis of pulp tissues from clinically healthy impacted third molars in 17 patients. Samples from coronal pulp, outer radicular dentin, and negative controls were included. Each sample was spiked with a known bacterial standard. Sequencing of the 16S rRNA V3–V4 region revealed similarity in bacterial taxonomic profiles. The bacterial DNA was detected in all pulp samples, primarily originating from reagent contaminants. Although some oral taxa appeared more abundant in pulp than in dentin or controls, contaminant species overwhelmingly dominated. Most of the extracted DNA was of human origin, rather than bacterial. Our findings question the concept of a core microbiome in healthy pulp and highlight the critical need for rigorous controls in pulpal microbiome studies. Microbiota Metataxonomics 16S ribosomal RNA Next-generation sequencing Spike-in controls Dental pulp qPCR quantification Dewey 617.6 Dewey 616 Dewey 617 205 Division de cariologie et d'endodontie 24P1 56 Département de médecine 221 57 Département de chirurgie 222 false 338 Génomique et microbiote 604 508 Groupe Bouillaguet Serge (medecine dentaire) 846 https://www.ebi.ac.uk/ena/browser/view/PRJEB50897 International journal of molecular sciences 26 7 119eb886-7ede-4744-90bf-83800b7babdf Article (Published version) Public ijms-26-03232.pdf 2323062 application/pdf CC BY-4.0 7119d40214c67c8fee40eb4b180bd3e938610f45c7f0df23a4576ccf5ceeaae9 openAccess Faculté de médecine / Section de médecine dentaire / Département de médecine dentaire préventive et de premier recours / Division de cariologie et d'endodontie Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine clinique / Département de chirurgie

Title	Published in	Year	Views	Downloads

Analyse omique du microbiote commensal intrapulpaire		2025	46	74
Microbiota of healthy dental pulps under the omics loupe	International journal of molecular sciences	2025	102	290

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Berard, Alan

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