Nucleolytic Inactivation and Degradation of the RNase III Processed pnp Message Encoding Polynucleotide Phosphorylase of Escherichia coli

Article Article scientifique Article Nucleolytic Inactivation and Degradation of the RNase III Processed pnp Message Encoding Polynucleotide Phosphorylase of Escherichia coli eng 1994-06 author Hajnsdorf Eliane CNRS URA1139, Institut de Biologie Physico-chimique, Paris, France. author Carpousis Agamemnon James 23343 author Regnier Philippe Thierry 74898 439-454 10.1006/jmbi.1994.1387 7516438 0022-2836 https://linkinghub.elsevier.com/retrieve/pii/S0022283684713878 The two cleavages made by RNase III in the transcripts of the pnp gene of Escherichia coli, 80 nucleotides upstream of the coding sequence of polynucleotide phosphorylase, were previously demonstrated to trigger the rapid degradation of the pnp messenger. In this paper, we demonstrate that the 5' end of the RNase III processed pnp mRNA is attacked by ribonucleases more efficiently than the rest of the molecule. Several 5' extremities resulting from cleavages occurring in the first 500 nucleotides of the pnp transcript have been identified. Three of them referred to as X, Y and W occur in the wild-type strain at the beginning of the coding sequence of the pnp mRNA. The mRNA appears to be cleaved more efficiently at the X site, proximal to the initiation codon, than at sites Y and W located downstream. In vitro, the maturation at X is catalysed by RNase E but not by RNase III. Accumulation of RNA processed at X in RNase E deficient strains leads us to postulate that X is a high affinity primary site which is slowly cleaved by the residual activity of thermosensitive RNase E at non-permissive temperature and that secondary sites located downstream are processed less efficiently than X. Taken together, our results suggest that in wild-type E. coli the degradation of the RNase III processed mRNA is mediated by RNase E. Escherichia coli Escherichia coli Proteins Molecular Sequence Data Nucleic acids Nucleotide sequence Dewey 570 43 Département de biologie moléculaire 161 false Journal of Molecular Biology 239 4 63d8a409-2e58-47cd-9ff9-563b15d11a27 Article (Published version) Restricted 1-s2.0-S0022283684713878-main.pdf 3925568 application/pdf e596b85acf943005bec9f3a3ee46cd439fe13d82ba447d50ec2e38462f8c988f restrictedAccess Faculté des sciences / Section de biologie / Département de biologie moléculaire

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Nucleolytic Inactivation and Degradation of the RNase III Processed pnp Message Encoding Polynucleotide Phosphorylase of Escherichia coli	Journal of Molecular Biology			1994	12	0

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Regnier, Philippe Thierry