MS/MS N-terminome Analysis and Identification of Protease Cleavage Products

Diplôme Master MS/MS N-terminome Analysis and Identification of Protease Cleavage Products eng 2012 author Campbell Nottania 724802 director Mueller Markus 767011 director Lisacek Frédérique 0000-0002-0948-4537 639315 Here a workflow is proposed for the improved identification of proteolytic cleavage products using firstly a combination of peptide mass search engines that maximizes the number of positively identified spectra corresponding to a cleavage event. Then, the output is combined and used for running a subsequent spectrum library search for the detection of possible post-translational modifications. Using this workflow we identified 3,804 unique peptides and 5,935 spectra corresponding to 107 additional Matrix Metalloproteinase (MMP) 2 cleavage substrates, 5 neo N-termini generated exclusively by MMP-9, and 27 substrates shared by both MMP-2 and MMP-9. Additionally, we identified amino acids (AA) at the cleavage sites prone to missed cleavage or addition; all of which were not previously reported in a prior publication from which the initial data was derived. Implementing this workflow overcomes the issues one may face whilst combining results obtained from multiple sequence database search tools and creating spectrum libraries. Dewey 570 Dewey 610 Maitrise Universitaire en Biologie orientation BIADB 30 Section de biologie 16 3 Faculté de médecine 2 false 405b9ec3-ed88-49e8-9c17-4f861cf66d82 Swiss Institute of Bioinformatics (SIB) ac0d8868-e74c-4197-a36f-9ac3b6d15d62 Master thesis Restricted CAMPBELL_Nottania_2012BIOLOGY_BIADB.pdf 3836714 application/pdf restrictedAccess Faculté des sciences / Section de biologie Faculté de médecine

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MS/MS N-terminome Analysis and Identification of Protease Cleavage Products				2012	443	0

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Campbell, Nottania