Retinal patterning by Pax6-dependent cell adhesion molecules

Article Article scientifique Article Retinal patterning by Pax6-dependent cell adhesion molecules eng 2010 author Rungger-Brändle Elisabeth author Ripperger Jürgen A. author Steiner Kurt author Conti Alain 723126 author Stieger Ariane 512823 author Soltanieh Sahar 525619 author Rungger Duri 25122 764-780 10.1002/dneu.20816 1932-8451 Long-standing evidence gained from Pax6 mutant embryos pointed to an involvement of Pax6-dependent cell adhesion molecules in patterning the central nervous system and, in particular, the retina. However, direct evidence for such pathways remained elusive. We here present direct evidence that knockdown of Pax6 expression by morpholino antisense molecules in Xenopus embryos and knockdown of maternal N-cadherin (mNcad), N-cadherin (Ncad) and neural cell adhesion molecule (NCAM) produce similar phenotypes. Eye formation is reduced and retinal lamination is heavily disorganized. In Pax6 knockdown embryos, the levels of mRNAs coding for these cell adhesion molecules are markedly reduced. Overexpression of Pax6 efficiently rescues the phenotype of Pax6 knockdown embryos and restores expression of these putative target genes. Rescue of Pax6-deficiency by the putative target gene mNcad moderately rescues eye formation. The promoters of the genes coding for cell adhesion molecules contain several putative Pax6 binding sites, as determined by computer analysis. Chromatin immunoprecipitation shows that, in embryonic heads, Pax6 binds to promoter regions containing such predicted binding sites. Thus, several cell adhesion molecules are direct target genes of Pax6 and cooperate in retinal patterning. Dewey 590 Dewey 616.8 44 Département de génétique et évolution 162 61 Département des neurosciences cliniques 226 false Developmental neurobiology 70 11 a2c47483-4bc7-4eba-a3b4-4af8d3a4d4de Article (Published version) Restricted Rungger-Brandle.pdf 1082716 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution Faculté de médecine / Section de médecine clinique / Département des neurosciences cliniques

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Retinal patterning by Pax6-dependent cell adhesion molecules	Developmental neurobiology			2010	782	0

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