Oxidative stress induced by sub-lethal exposure to copper as a mediator in development of bacterial resistance to antibiotics

Article Article scientifique Article Oxidative stress induced by sub-lethal exposure to copper as a mediator in development of bacterial resistance to antibiotics eng 2023-02 author Liu Wei 0000-0003-2018-7004 884892 Forel Communs (FOREL) author Xu Yang 715495 author Slaveykova Vera 0000-0002-8361-2509 510054 Forel Communs (FOREL) 160516 10.1016/j.scitotenv.2022.160516 36470380 0048-9697 https://linkinghub.elsevier.com/retrieve/pii/S0048969722076185 Limited information exists on how bacterial resistance to antibiotics is acquired and altered in response to short-term metal stress, and what the prevailing pathways are. Here the precursor mechanisms of development of bacterial antibiotic resistance mediated by <a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/oxidative-stress" rel="noopener noreferrer" target="_blank">oxidative stress</a> induce under sub-lethal Cu2+ exposure were explored. The results showed that the overall level of antibiotic resistance in wild-type Escherichia coli and antibiotic-resistant E. coli was enhanced under 4 and 20 mg/L Cu2+ exposure, as demonstrated by the 2- to 8-fold increase in minimum inhibitory concentration (MIC). The MIC correlated with the increase of the cellular ROS generation and the enhancement of the antioxidant <a href="https://www.sciencedirect.com/topics/earth-and-planetary-sciences/catalytic-activity" rel="noopener noreferrer" target="_blank">enzyme activity</a> (p < 0.05), suggesting that changes in antibiotic resistance under sub-lethal Cu2+ exposure could be associated with oxidative stress. Likewise, enhanced cell membrane permeability and an increase in the number of bacteria entering the viable but non culturable (VBNC) state contributed to bacterial resistance to antibiotics. Moreover, the variance partitioning analysis demonstrated that the alterations of the antibiotic resistance phenotype of wild-type E. coli was mainly caused by oxidative stress-mediated increase in cell membrane permeability and entry into the VBNC state. The development of antibiotic resistance in resistant E. coli was primarily attributed to changes in the abundance and horizontal transfer ability of its antibiotic resistance genes, both of which contributed up to 20 %. Taken together the results allowed to propose a conseptual scheme on developing bacterial antibiotic resistance mediated by oxidative stress under sub-lethal Cu2+ exposure. This result provided a strong basis for reduction of early bacterial resistance. Antibiotic resistance Cell membrane Cooper Oxidative stress Dewey 550 Dewey 333.7-333.9 41 Département F.-A. Forel des sciences de l'environnement et de l'eau 153 18 Institut des sciences de l'environnement 0DB false 752 Environmental Biogeochemistry and Ecotoxicology 938 ISE Pôle Sciences 944 ISE Eau Science of the total environment 860 f994c621-8d65-4ee1-aada-c7deea5e8c20 Article (Published version) Restricted 1-s2.0-S0048969722076185-main.pdf 2452795 application/pdf ce72bb8060cf806a6a5f59d68cb21c126deabb27bece315c8a93e325c155fc7e restrictedAccess Faculté des sciences / Section des sciences de la Terre et de l'Environnement / Département F.-A. Forel des sciences de l'environnement et de l'eau Centres et instituts / Institut des sciences de l'environnement

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Oxidative stress induced by sub-lethal exposure to copper as a mediator in development of bacterial resistance to antibiotics	Science of the total environment			2023	141	2

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