Postnatal distribution of cpp32/caspase 3 mRNA in the mouse central nervous system: an in situ hybridization study

Article Article scientifique Article Postnatal distribution of cpp32/caspase 3 mRNA in the mouse central nervous system: an in situ hybridization study eng 1999 author De Bilbao Fabienne 91197 author Guarin Ernesto 87900 author Nef Patrick 22181 author Vallet Pierre Luc 70728 author Giannakopoulos Panteleimon 44657 Département de psychiatrie (PSYAT) author Dubois-Dauphin Michel 21885 339-357 10.1002/(SICI)1096-9861(19990705)409:3<339 0021-9967 Apoptotic cell death is a major feature of the developing nervous system and of certain neurodegenerative diseases. Various gene effectors and repressors of this type of cell death have been identified. Among them, bcl-xl and bax, which encode for antiapoptotic and proapoptotic proteins, respectively, play major roles during development. The gene cpp32 encodes for the caspase 3 cysteine protease and is a critical mediator of cell death during embryonic development in the mammalian brain. To gain insight into the possible implications of these cell death genes during the postnatal development, we investigated the expression of bax, bcl-xl, and cpp32 mRNAs by in situ hybridization in the mouse brain from birth to adulthood. Whereas bax and bcl-xl mRNAs were expressed widely in neonates and adult mice, our results showed that cpp32 mRNA levels were decreased strongly from 12 postnatal days. From 1 postnatal day to 12 postnatal days, cpp32 mRNA was expressed ubiquitously in all brain nuclei, including areas where neurogenesis occured. A positive correlation between areas displaying high levels of mRNA and apoptotic nuclei also was shown. In the adult, cpp32 mRNA was restricted to the piriform and entorhinal cortices, the neocortex, and to areas where neurogenesis is observed (e.g., olfactory bulb and dentate gyrus). The same pattern of expression was observed in adult mice over-expressing the antiapoptotic protein Bcl-2. These results demonstrate that the expression of cpp32 mRNA is highly regulated during the mouse postnatal period, leading to a specific distribution in the adult central nervous system. Moreover, the prevention of cell death by Bcl-2 likely is not linked to the regulation of caspase mRNA levels. Apoptosis Brain Caspase In situ hybridization Cell death genes Bcl-2 Dewey 540 Dewey 616.89 60 Département de psychiatrie 225 39 Département de biochimie 144 false Journal of comparative neurology 409 3 b1e552a8-9cbf-44ca-b8dc-21c1b53f7f2f Article (Published version) Restricted Bilbao_TJCN_1999357.pdf 1485344 application/pdf f10915d9162b01d7877da3c2fbe44f0c39acfac0df452ba7d7c66e281f29602c restrictedAccess Faculté de médecine / Section de médecine clinique / Département de psychiatrie Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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Postnatal distribution of cpp32/caspase 3 mRNA in the mouse central nervous system: an in situ hybridization study	Journal of comparative neurology			1999	50	0

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Vallet, Pierre Luc