Role of proteomics to differentiate between benign and potentially malignant pancreatic cysts

Article Article scientifique Article Role of proteomics to differentiate between benign and potentially malignant pancreatic cysts eng 2011 author Cuoghi Aurora 699718 author Farina Annarita 649783 author Z'graggen Kaspar author Dumonceau Jean-Marc 565160 author Tomasi Aldo author Hochstrasser Denis 26422 author Genevay-Infante Muriel 628497 author Lescuyer Pierre 0000-0001-6986-8738 562350 author Frossard Jean-Louis 35038 2664-2670 10.1021/pr2000557 21425880 1535-3893 Pancreatic cystic neoplasms represent 10-15% of primary cystic masses of the pancreas. While pancreatic cysts are detected with an increasing frequency due to the use of advanced imaging modalities in clinical practice, the diagnosis of pancreatic cystic neoplasms remains unsatisfactory because available diagnostic techniques proved not sensitive enough so far. This study was designed to characterize the proteomic pattern of pancreatic cyst fluids obtained from various cystic lesions. Cyst fluids were collected by direct puncture during open surgery to avoid any possible contamination from other tissues. CEA, CA-19-9, and amylase concentrations were measured using specific immunoassays. After immunodepletion and fractionation by SDS-PAGE, proteins were digested and analyzed by LC-MS/MS. Specific histological lesions were found to be associated with distinct protein patterns. Interestingly, some of these proteins have been proposed as biomarkers of pancreatic cancer. Immunoblots allowed for verifying the differential expression in specific cyst fluids of two selected proteins, olfactomedin-4 and mucin-18. Finally, immunohistochemistry was performed to correlate these data with the expression pattern of olfactomedin-4 and mucin-18 in pancreatic cyst tissues. Results from this study indicate that proteomic analysis of cyst fluid could provide reliable candidates for developing new biomarkers for the preoperative management of malignant and premalignant pancreatic cysts. Amylases/analysis CA-19-9 Antigen/analysis Carcinoembryonic Antigen/analysis Chromatography, Liquid Cyst Fluid/chemistry Electrophoresis, Polyacrylamide Gel Granulocyte Colony-Stimulating Factor/metabolism Humans Immunoassay Immunoblotting Immunohistochemistry Mucins/metabolism Pancreatic Cyst/diagnosis/metabolism Pancreatic Neoplasms/diagnosis/metabolism Proteomics/methods Tandem Mass Spectrometry Tumor Markers, Biological/analysis Dewey 576 Dewey 616 Dewey 616.07 136 Département de biologie structurale et bioinformatique 21E 137 Département de pathologie et immunologie 21F 56 Département de médecine 221 false 211 Auto-immunité, inflammation et athérosclérose 270 391 Métastases du foie 657 335 Pancréatite aiguë 601 Journal of proteome research 10 5 cb2e9db1-879c-462b-a29b-d6bcfc1a9978 Article (Published version) Restricted Genevay M J Proteome Res 2011, 10,2664.pdf 924903 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de médecine

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Role of proteomics to differentiate between benign and potentially malignant pancreatic cysts	Journal of proteome research			2011	662	0

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Cuoghi, Aurora