Mitochondrial translation in absence of local tRNA aminoacylation and methionyl tRNA formylation in Apicomplexa

Article Article scientifique Article Mitochondrial translation in absence of local tRNA aminoacylation and methionyl tRNA formylation in Apicomplexa eng 2010 author Pino Paco 629465 author Aeby Eric author Foth Bernardo Javier 619237 author Sheiner Lilach author Soldati Thierry 0000-0002-2056-7931 135632 author Schneider Andréas 698954 author Soldati-Favre Dominique 0000-0003-4156-2109 135114 706-718 10.1111/j.1365-2958.2010.07128.x 20374492 0950-382X Summary Apicomplexans possess three translationally active compartments: the cytosol, a single tubular mitochondrion, and a vestigial plastid organelle called apicoplast. Mitochondrion and apicoplast are of bacterial evolutionary origin and therefore depend on a bacterial-like translation machinery. The minimal mitochondrial genome contains only three ORFs, and in Toxoplasma gondii the absence of mitochondrial tRNA genes is compensated for by the import of cytosolic eukaryotic tRNAs. Although all compartments require a complete set of charged tRNAs, the apicomplexan nuclear genomes do not hold sufficient aminoacyl-tRNA synthetase (aaRSs) genes to be targeted individually to each compartment. This study reveals that aaRSs are either cytosolic, apicoplastic or shared between the two compartments by dual targeting but are absent from the mitochondrion. Consequently, tRNAs are very likely imported in their aminoacylated form. Furthermore, the unexpected absence of tRNA(Met) formyltransferase and peptide deformylase implies that the requirement for a specialized formylmethionyl-tRNA(Met) for translation initiation is bypassed in the mitochondrion of Apicomplexa. Dewey 540 Dewey 616 39 Département de biochimie 144 54 Département de microbiologie et médecine moléculaire 21B false 443 Biologie d'un parasite intracellulaire obligatoire 773 Molecular microbiology 76 3 fcb69f15-ba31-49f2-b5d8-742475f11b96 Article (Published version) Restricted fulltext.pdf 935661 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Mitochondrial translation in absence of local tRNA aminoacylation and methionyl tRNA formylation in Apicomplexa	Molecular microbiology			2010	630	0

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Schneider, Andréas