Peripheral Deafferentation-Driven Functional Somatosensory Map Shifts Are Associated with Local, Not Large-Scale Dendritic Structural Plasticity

Article Article scientifique Article Peripheral Deafferentation-Driven Functional Somatosensory Map Shifts Are Associated with Local, Not Large-Scale Dendritic Structural Plasticity eng 2013 author Schubert Vanessa 691904 author Lebrecht Daniel 718063 author Holtmaat Anthony 0000-0002-7577-0769 661031 9474-9487 10.1523/JNEUROSCI.1032-13.2013 23719814 0270-6474 Long-term peripheral deafferentation induces representational map changes in the somatosensory cortex. It has been suggested that dendrites and axons structurally rearrange in such paradigms. However, the extent and process of this plasticity remains elusive. To more precisely quantify deafferentation-induced structural plasticity of excitatory cells we repeatedly imaged GFP-expressing L2/3 and L5 pyramidal dendrites in the mouse barrel cortex over months after the removal of a subset of the whisker follicles (FR), a procedure that completely and permanently removes whisker-sensory input. In the same mice we imaged whisker-evoked intrinsic optical signals (IOS) to assess functional cortical map changes. FR triggered the expansion of spared whisker IOS responses, whereas they remained unchanged over months in controls. The gross structure and orientation of apical dendrite tufts remained stable over a two-month period, both in controls and after deprivation. However, terminal branch tip dynamics were slightly reduced after FR, and the formation of new dendritic spines was increased in a cell-type and location-dependent manner. Together, our data suggest that peripheral nerve lesion-induced cortical map shifts do not depend on the large scale restructuring of dendritic arbors but are rather associated with local cell-type and position-dependent changes in dendritic synaptic connectivity. Dewey 616.8 53 Département de neurosciences fondamentales 21A false 517 Les circuits neuronaux dans le néocortex des mammifères 855 The Journal of neuroscience 33 22 e19a39dc-0033-4972-a61a-6789be7a493f Article (Accepted version) Restricted Schubert_Holtmaat_JN_small.pdf 1864788 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales

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Peripheral Deafferentation-Driven Functional Somatosensory Map Shifts Are Associated with Local, Not Large-Scale Dendritic Structural Plasticity	The Journal of neuroscience			2013	609	0

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Schubert, Vanessa