The role of interstitial fluid pressure in cerebral porous biomaterial integration

Article Article scientifique Article The role of interstitial fluid pressure in cerebral porous biomaterial integration eng 2022-03-22 2022-03-22 author Bonini Fabien Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, CH-1022 Geneva, Switzerland. 863519 Département de pathologie et immunologie (PATIM) author Mosser Sébastien Neurix SA, Avenue de la Roseraie 64, CH-1022 Geneva, Switzerland. 911997 author Mor Flavio Maurizio Haute École du Paysage, d'Ingénierie et d'Architecture de Genève, Haute École Spécialisée de Suisse Occidentale (HEPIA HES-SO), University of Applied Sciences and Arts Western Switzerland, CH-1202 Geneva, Switzerland. author Boutabla Anissa Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, CH-1022 Geneva, Switzerland. 872142 Services communs du département des neurosciences cliniques (SPECO) author Burch Patrick Volumina-Medical SA, Route de la Corniche 5, CH-1066 Epalinges, Switzerland. author Beduer Amélie Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, CH-1022 Geneva, Switzerland.,Volumina-Medical SA, Route de la Corniche 5, CH-1066 Epalinges, Switzerland. 862936 author Roux Adrien 0000-0001-7618-6129 Haute École du Paysage, d'Ingénierie et d'Architecture de Genève, Haute École Spécialisée de Suisse Occidentale (HEPIA HES-SO), University of Applied Sciences and Arts Western Switzerland, CH-1202 Geneva, Switzerland. 691460 author Braschler Thomas Michaël 0000-0003-1811-1713 Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, CH-1022 Geneva, Switzerland. 848483 417 10.3390/brainsci12040417 35447953 2076-3425 PMC9040716 https://www.mdpi.com/2076-3425/12/4/417 Recent advances in biomaterials offer new possibilities for brain tissue reconstruction. Biocompatibility, provision of cell adhesion motives and mechanical properties are among the present main design criteria. We here propose a radically new and potentially major element determining biointegration of porous biomaterials: the favorable effect of interstitial fluid pressure (IFP). The force applied by the lymphatic system through the interstitial fluid pressure on biomaterial integration has mostly been neglected so far. We hypothesize it has the potential to force 3D biointegration of porous biomaterials. In this study, we develop a capillary hydrostatic device to apply controlled in vitro interstitial fluid pressure and study its effect during 3D tissue culture. We find that the IFP is a key player in porous biomaterial tissue integration, at physiological IFP levels, surpassing the known effect of cell adhesion motives. Spontaneous electrical activity indicates that the culture conditions are not harmful for the cells. Our work identifies interstitial fluid pressure at physiological negative values as a potential main driver for tissue integration into porous biomaterials. We anticipate that controlling the IFP level could narrow the gap between in vivo and in vitro and therefore decrease the need for animal screening in biomaterial design. Biointegration Biomaterial Glymphatic system Interstitial fluid Dewey 616.07 Swiss National Science Foundation Brain Patch Technology 163684 CH Careers Swiss National Science Foundation In vivo perfused and stimulated scaffolds for neural cell therapy 161347 CH Careers Swiss National Science Foundation Cell Patch Technology 194813 CH Careers 137 Département de pathologie et immunologie 21F false 972 Groupe Thomas Braschler 970 http://doi.org/10.5281/zenodo.5115814 https://doi.org/10.24433/CO.7598976.v1 https://doi.org/10.5281/zenodo.4589276 https://github.com/tbgitoo/textureAnalyzerGels https://doi.org/10.5281/zenodo.4589498 https://github.com/tbgitoo/plot.counts https://doi.org/10.5281/zenodo.4594515 https://github.com/tbgitoo/reproducibleCalculationTools Brain sciences 12 4 70bec6dd-e811-4400-bb65-518358e7cca3 Article (Published version) Public Article-scientifique-from-europePmc.pdf 3003624 application/pdf CC BY-4.0 7721d97de1caa24125a183ba892418a574cfaf5816510d78b3ccff5f07de7f85 96c4a6d3-d57a-488c-a796-e120b478fff6 Appendix Public brainsci-12-00417-s001.zip 548886 application/zip b906ca538c24c2fb4226fdd388d4d9cffd4856165fc709031571cc20906750ad openAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

Title	Published in	Year	Views	Downloads

The role of interstitial fluid pressure in cerebral porous biomaterial integration	Brain sciences	2022	97	62
Search for an antibody-based vehicle for delivering therapeutic molecules into the central nervous system		2014	908	127

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Roux, Adrien