Gnomoniopsis smithogilvyi causes chestnut canker symptoms in Castanea sativa shoots in Switzerland

Article Article scientifique Article Gnomoniopsis smithogilvyi causes chestnut canker symptoms in Castanea sativa shoots in Switzerland eng 2016-02 author Pasche Sabrina 684300 author Calmin Gautier 0000-0001-5508-7922 619399 author Auderset Guy Leon 24662 author Crovadore Julien author Pelleteret Pegah author Mauch-Mani Brigitte author Barja François 26490 author Paule Bernard 0000-0002-3640-6235 28279 author Jermini Mauro author Lefort François 22463 9-21 10.1016/j.fgb.2016.01.002 26768710 1087-1845 https://linkinghub.elsevier.com/retrieve/pii/S1087184516300019 A screening of Castanea sativa scions for grafting for the presence of endophytes showed that the opportunistic fungal pathogen Gnomoniopsis smithogilvyi was the most abundant member of the endophytic flora. This fungus is known as a pathogen affecting chestnut fruits in Italy and Australia. Here, we present evidence that it causes cankers very similar to the ones due to Cryphonectria parasitica infection on twigs and scions of chestnut trees. We found natural infections of G. smithogilvyi in healthy grafted plants as well as in scions from chestnut trees. The identity of the fungus isolated from asymptomatic tissues was verified by applying Koch’s postulates and corroborated by DNA sequencing of four different gene regions. In contrast to C. parasitica that appears on the bark as yellow to orange pycnidia, stromata and slimy twisted tendrils, G. smithogilvyi forms orange to red and black pycnidia, gray stromata and cream-colored to beige slimy twisted tendrils on the bark. These Swiss strains are closely related to G. smithogilvyi strains from Australia and from New Zealand, Gnomoniopsis sp. and Gnomoniopsis castanea from New Zealand, Italy, France and Switzerland. While the strains from Ticino are genetically very close to G. smithogilvyi and G. castanea from Italy, the differences between the strains from Ticino and Geneva suggest two different origins. The present study supports the hypothesis that a single species named G. smithogilvyi, which is known to be the agent of chestnut rot, also causes wood cankers on chestnut. Castanea sativa Chestnut blight Chestnut rot Cryphonectria parasitica Endophyte Gnomoniopsis smithogilvyi Dewey 580 45 Département des sciences végétales 163 false Fungal genetics and biology 87 ae632fb0-6c7b-4cf0-bbdf-e6984f66d081 Article (Published version) Restricted BARJA02.pdf 413900 application/pdf 197486561a8f95dd87f83cf716ad89593fa3cd3c3ffba03bafb336c3bc11f509 restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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Gnomoniopsis smithogilvyi causes chestnut canker symptoms in Castanea sativa shoots in Switzerland	Fungal genetics and biology			2016	68	0

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