Membrane remodeling induced by the dynamin-related protein Drp1 stimulates Bax oligomerization

Article Article scientifique Article Membrane remodeling induced by the dynamin-related protein Drp1 stimulates Bax oligomerization eng 2010 author Montessuit Sylvie 134333 author Somasekharan Syam Prakash 650362 author Terrones Urio Oihana 680248 author Lucken-Ardjomande Safa 507264 author Herzig Sébastien 552049 author Schwarzenbacher Robert author Manstein Dietmar J. author Bossy-Wetzel Ella author Basañez Gorka author Meda Paolo 26346 author Martinou Jean-Claude 27293 889-901 10.1016/j.cell.2010.08.017 20850011 1097-4172 In response to many apoptotic stimuli, oligomerization of Bax is essential for mitochondrial outer membrane permeabilization and the ensuing release of cytochrome c. These events are accompanied by mitochondrial fission that appears to require Drp1, a large GTPase of the dynamin superfamily. Loss of Drp1 leads to decreased cytochrome c release by a mechanism that is poorly understood. Here we show that Drp1 stimulates tBid-induced Bax oligomerization and cytochrome c release by promoting tethering and hemifusion of membranes in vitro. This function of Drp1 is independent of its GTPase activity and relies on arginine 247 and the presence of cardiolipin in membranes. In cells, overexpression of Drp1 R247A/E delays Bax oligomerization and cell death. Our findings uncover a function of Drp1 and provide insight into the mechanism of Bax oligomerization. Amino Acid Sequence Animals Apoptosis BH3 Interacting Domain Death Agonist Protein/metabolism Cardiolipins/metabolism Cell-Free System GTP Phosphohydrolases/metabolism HeLa Cells Humans Liposomes/metabolism Microtubule-Associated Proteins/metabolism Mitochondrial Membranes/metabolism Mitochondrial Proteins/metabolism Models, Molecular Molecular Sequence Data Rats Bcl-2-Associated X Protein/metabolism Dewey 570 46 Département de biologie cellulaire 165 false Cell 142 6 a5c2df9b-fb16-40fb-bfe2-56fb74a3157b Article (Published version) Public membrane Remo.pdf 1406432 application/pdf openAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Membrane remodeling induced by the dynamin-related protein Drp1 stimulates Bax oligomerization	Cell			2010	813	699

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