Large-scale transcriptional profiling of chemosensory neurons identifies receptor-ligand pairs in vivo

Article Article scientifique Article Large-scale transcriptional profiling of chemosensory neurons identifies receptor-ligand pairs in vivo eng 2015 author Von Der Weid Benoît 643815 author Rossier Daniel 583687 author Lindup Matti 676809 author Tuberosa Joël 0000-0001-6022-0556 644501 author Widmer Alexandre 741073 author Dal Col Julien 515208 author Kan Chenda author Carleton Alan 676577 author Rodriguez Ivan 28183 1455-1463 10.1038/nn.4100 26322926 1097-6256 In mammals, olfactory perception is based on the combinatorial activation of G protein–coupled receptors. Identifying the full repertoire of receptors activated by a given odorant in vivo, a quest that has been hampered for over 20 years by technical difficulties, would represent an important step in deciphering the rules governing chemoperception. We found that odorants induced a fast and reversible concentration-dependent decrease in the transcription of genes corresponding to activated receptors in intact mice. On the basis of this finding, we developed a large-scale transcriptomic approach to uncover receptor-ligand pairs in vivo. We identified the mouse and rat odorant receptor signatures corresponding to specific odorants. Finally, we found that this approach, which can be used for species for which no genomic sequence is available, is also applicable to non-vertebrate species such as Drosophila. Dewey 616.8 Dewey 590 European Commission From neurons to behavior: analysis of the mechanisms underlying sensory coding and plasticity in chemical senses 243344 NEUROCHEMS EU FP7 Swiss National Science Foundation Emergence and regulation of vomeronasal receptor genes in the mouse 149753 CH Projects Swiss National Science Foundation A novel olfactory sensory population 143723 CH Projects Swiss National Science Foundation Does ensemble decorrelation in the olfactory bulb predict odor discrimination behavior? 153410 CH Projects 44 Département de génétique et évolution 162 53 Département de neurosciences fondamentales 21A false 536 Groupe Carleton Alan (neurosciences fondamentales) 876 820 Groupe Rodriguez Nature neuroscience 18 10 e8c4d141-3d15-41c1-8ece-09d3fa26cebc Article (Published version) Restricted von der weidNatNeurosci2015.pdf 2654824 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de génétique et évolution Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales

Title	Published in	Year	Views	Downloads

Transcriptional profiling as a novel approach to determining receptor-ligand pairs in the mouse olfactory system		2015	290	29
Large-scale transcriptional profiling of chemosensory neurons identifies receptor-ligand pairs in vivo	Nature neuroscience	2015	779	11

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