Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation

Article Article scientifique Article Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation eng 2015 author Chiaruttini Nicolas 743514 author Redondo-Morata Lorena author Colom Diego Adai 815331 author Humbert Frédéric Jean-Christian 730215 author Lenz Martina 672513 author Scheuring Simon author Roux Aurélien 0000-0002-6088-0711 716946 866-879 10.1016/j.cell.2015.10.017 0092-8674 https://linkinghub.elsevier.com/retrieve/pii/S0092867415013318 ESCRT-III is required for lipid membrane remodeling in many cellular processes, from abscission to viral budding and multi-vesicular body biogenesis. However, how ESCRT-III polymerization generates membrane curvature remains debated. Here, we show that Snf7, the main component of ESCRT-III, polymerizes into spirals at the surface of lipid bilayers. When covering the entire membrane surface, these spirals stopped growing when densely packed: they had a polygonal shape, suggesting that lateral compression could deform them. We reasoned that Snf7 spirals could function as spiral springs. By measuring the polymerization energy and the rigidity of Snf7 filaments, we showed that they were deformed while growing in a confined area. Furthermore, we observed that the elastic expansion of compressed Snf7 spirals generated an area difference between the two sides of the membrane and thus curvature. This spring-like activity underlies the driving force by which ESCRT-III could mediate membrane deformation and fission. Dewey 540 39 Département de biochimie 144 false Cell 163 4 3651b142-c332-467a-b055-a87bf1fb2c7c Article (Published version) Public Relaxation_HR_15.pdf 7298427 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation	Cell			2015	197	78

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Lenz, Martina