TiN x O y coatings facilitate the initial adhesion of osteoblasts to create a suitable environment for their proliferation and the recruitment of endothelial cells

Article Article scientifique Article TiN x O y coatings facilitate the initial adhesion of osteoblasts to create a suitable environment for their proliferation and the recruitment of endothelial cells eng 2017 author Moussa Mira 782320 author Banakh O author Wehrle-Haller Bernhard 0000-0002-1159-1147 116298 author Fontana Pierre 135328 author Scherrer Susanne 22647 author Cattani Manon 672050 author Wiskott Anselm 26491 author Durual Stéphane 0000-0002-9772-8406 592156 025001 10.1088/1748-605X/aa57a7 28244429 1748-6041 Titanium-nitride-oxide coatings (TiN x O y ) improve osseointegration of endosseous implants. The exact mechanisms by which these effects are mediated are poorly understood except for an increase of osteoblast proliferation while a high degree of differentiation is maintained. One hypothesis holds that TiN x O y facilitates the initial spreading and adhesion of the osteoblasts. The aim of this work was to investigate the molecular mechanisms of osteoblast adhesion on TiN x O y as compared to microrough titanium SLA. A global view of the osseointegrative process, that is, taking into account other cell groups, especially endothelial cells, is also presented. To this aim, gene expression and focal adhesion analysis, cocultures and wound assays were performed early after seeding, from 6 h to 3 days. We demonstrated that TiN x O y coatings enhance osteoblast adhesion and spreading when compared to the standard microrough titanium. The integrin β1, either in association with α1 or with α2 plays a central role in these mechanisms. TiN x O y coatings optimize the process of osseointegration by acting at several levels, especially by upregulating osteoblast adhesion and proliferation, but also by supporting neovascularization and the development of a suitable inflammatory environment. Dewey 616 Dewey 617.6 56 Département de médecine 221 210 Division de prothèse fixe et biomatériaux 24Q2 false 11 Geneva Platelet Group 13 239 Groupe Susanne Scherrer : Unité des biomatériaux 315 379 Migration cellulaire 645 Biomedical materials 12 2 49efefda-31b1-472a-86c9-9f83fad36df8 Article (Published version) Restricted Moussa_2017_Biomed._Mater._12_025001.pdf 3838915 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine dentaire / Département de réhabilitation oro-faciale / Division de prothèse fixe et biomatériaux

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TiN x O y coatings facilitate the initial adhesion of osteoblasts to create a suitable environment for their proliferation and the recruitment of endothelial cells	Biomedical materials			2017	731	2

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