Identification of Chlamydomonas central core centriolar proteins reveals a role for human wdr90 in ciliogenesis

Article Article scientifique Article Identification of Chlamydomonas central core centriolar proteins reveals a role for human wdr90 in ciliogenesis eng 2017-08-21 author Hamel Virginie 0000-0001-5092-2343 846734 Département de biologie moléculaire et cellulaire (MOCEL) author Steib Emmanuelle 0000-0002-5897-1964 847003 author Hamelin Romain 666617 author Armand Florence 528967 author Borgers Susanne 701835 Département de biologie moléculaire et cellulaire (MOCEL) author Fluckiger Isabelle 132968 author Busso Coralie author Olieric Natacha 0000-0002-6273-390X author Sorzano Carlos Oscar S. author Steinmetz Michel O. author Guichard Paul 0000-0002-0363-1049 845268 Département de biologie moléculaire et cellulaire (MOCEL) author Gonczy Pierre 742409 2486-2498.e6 10.1016/j.cub.2017.07.011 28781053 0960-9822 PMC6399476 https://linkinghub.elsevier.com/retrieve/pii/S0960982217308692 Centrioles are evolutionarily conserved macromolecular structures that are fundamental to form cilia, flagella, and <a href="https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/centrosome" rel="noopener noreferrer" target="_blank">centrosomes</a>. Centrioles are 9-fold symmetrical microtubule-based cylindrical barrels comprising three regions that can be clearly distinguished in the Chlamydomonas reinhardtii <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/organelle" rel="noopener noreferrer" target="_blank">organelle</a>: an ∼100-nm-long proximal region harboring a cartwheel; an ∼250-nm-long central core region containing a Y-shaped linker; and an ∼150-nm-long distal region ending at the transitional plate. Despite the discovery of many centriolar components, no protein has been localized specifically to the central core region in Chlamydomonas thus far. Here, combining relative quantitative <a href="https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/mass-spectrometry" rel="noopener noreferrer" target="_blank">mass spectrometry</a> and super-resolution microscopy on purified Chlamydomonas centrioles, we identified POB15 and POC16 as two proteins of the central core region, the distribution of which correlates with that of tubulin glutamylation. We demonstrated that POB15 is an inner barrel protein within this region. Moreover, we developed an assay to uncover temporal relationships between centriolar proteins during organelle assembly and thus established that POB15 is recruited after the cartwheel protein CrSAS-6 and before tubulin glutamylation takes place. Furthermore, we discovered that two poc16 mutants exhibit flagellar defects, indicating that POC16 is important for flagellum biogenesis. In addition, we discovered that WDR90, the human homolog of POC16, localizes to a region of human centrioles that we propose is analogous to the central core of Chlamydomonas centrioles. Moreover, we demonstrate that WDR90 is required for ciliogenesis, echoing the findings in Chlamydomonas. Overall, our work provides novel insights into the identity and function of centriolar central core components. Chlamydomonas Basal body Centriole Ciliogenesis Inner barrel Super-resolution microscopy Dewey 570 Swiss National Science Foundation Deciphering the structural mechanisms governing centriole and cilia formation 157517 CH Careers European Commission Dissecting the mechanisms governing centriole formation 340227 CENFOR EU FP7 46 Département de biologie cellulaire 165 false Current biology 27 16 4a141254-1784-471a-85a7-00146191aa40 Article (Published version) Public HAMEL13.pdf 7578100 application/pdf CC BY-NC-ND-4.0 2c545fe95b555f4493d533c4525c45b429ba5856900baf231c9c115f0bc2747f openAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

Title	Published in	Year	Views	Downloads

Identification of Chlamydomonas central core centriolar proteins reveals a role for human wdr90 in ciliogenesis	Current biology	2017	70	140
Connexins, diabetes and the metabolic syndrome	Current protein and peptide science	2009	539	0

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