Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of <i>Saccharomyces cerevisiae</i>

Article Article scientifique Article Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of <i>Saccharomyces cerevisiae</i> eng 1995-05 author Kraemer Angela Département de Biologie Cellulaire, Université de Genève, Switzerland. 22808 Département de biologie moléculaire et cellulaire (MOCEL) author Mulhauser Frank 22591 author Wersig Cornelia 66235 author Groening Karsten Johannes 88149 author Bilbe Graeme 260-272 7489498 1355-8382 PMC1369079 Mammalian splicing factor SF3a consists of three subunits of 60, 66, and 120 kDa and functions early during pre-mRNA splicing by converting the U2 snRNP into its active form. A cDNA encoding the 120-kDa subunit of SF3a has been cloned. The SF3a120 gene was localized to human chromosome 22, and three mRNAs of 3.2, 3.8, and 5.7 kb are ubiquitously expressed. The N-terminal half of the deduced SF3a120 amino acid sequence contains a tandemly repeated motif (the SURP module) that has recently been identified in the essential splicing factor PRP21p of Saccharomyces cerevisiae, the Drosophila alternative splicing regulator suppressor-of-white-apricot, and four proteins from nematodes and mammals; the C-terminal half is organized into a proline-rich region and a ubiquitin-like domain. The spacing between the SURP modules and the protein's essential function in constitutive splicing identify SF3a120 as the mammalian homologue of yeast PRP21p. Binding studies with truncated derivatives of SF3a120 revealed that the SURP domains function in binding to SF3a60, whereas a region of 130 amino acids C-terminal to these domains is essential for contacts with SF3a66. Amino Acid Sequence Animals Base Sequence Chromosome Mapping Chromosomes, Human, Pair 22 Cloning, Molecular Cricetinae DNA, Complementary / genetics Female Fungal Proteins / genetics Humans Male Mice Molecular Sequence Data Multigene Family Protein Binding Protein Conformation RNA Splicing RNA-Binding Proteins / genetics Repetitive Sequences, Nucleic Acid Ribonucleoprotein, U2 Small Nuclear Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Dewey 570 46 Département de biologie cellulaire 165 false RNA 1 3 1bac4dd4-a5e5-4d0d-ad40-f6991a54beef Article (Published version) Public Krämer_RNA_19951260.pdf 9308350 application/pdf CC BY-NC-4.0 0ac929422d09345ea19372ebc50e304b0d2c60d5bc2f3b35fc9d702e14f0a3fa openAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

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Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae	RNA			1995	93	43

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Wersig, Cornelia