Interactive effects of copper oxide nanoparticles and light to green alga Chlamydomonas reinhardtii

Article Article scientifique Article Interactive effects of copper oxide nanoparticles and light to green alga Chlamydomonas reinhardtii eng 2016 author Cheloni Giulia 764024 author Marti Elodie 658494 author Slaveykova Vera 0000-0002-8361-2509 510054 120-128 10.1016/j.aquatox.2015.11.018 0166-445X http://linkinghub.elsevier.com/retrieve/pii/S0166445X15301016 The present study explores the effect of light with different spectral composition on the stability of CuO-nanoparticle (CuO-NPs) dispersions and their effects to green alga Chlamydomonas reinhardtii. The results showed that simulated natural light (SNL) and light with enhanced UVB radiation (UVR*) does not affect the dissolution of CuO-NPs as compared to light irradiation conditions typically used in laboratory incubator (INC). Comparable values of -potential and hydrodynamic size during 24 h were found under all studied conditions. Concentrations of CuO-NPs below 1 mgL-1 do not attenuate the light penetration in the algal suspensions in comparison with NP-free system. Exposure to a combination of 8 gL-1 or 0.8 mgL-1 CuO-NPs and INC or SNL has no significant effect on the algal growth inhibition, algal fluorescence and membrane integrity under short-term exposure. However, an enhancement of the percentage of cells experiencing oxidative stress was observed upon exposure to 0.8 mgL-1 CuO-NPs and SNL for 4 and 8 hours. Combination of UVR* and 0.8 mg L-1 CuO-NPs resulted in synergistic effects for all biological endpoints. Despite the photocatalytic properties of CuO-NPs no significant increase in abiotic reactive oxygen species (ROS) production under simulated solar radiation were observed suggesting that the synergistic effect observed might be correlated to other factors than CuO-NPs mediated ROS photoproduction. Tests performed with CuSO4 confirmed the important role of dissolution as toxicity driving forse for lower CuO-NPs concentration, however failed to clarify the contribution of dissolved Cu on the combined effects at 0.8 mgL-1 CuO-NPs. The results point out the necessity of taking into account the possible interactions between ENPs and changing light conditions when evaluating the potential effects of ENPs to phytoplankton in natural waters. Nanoparticles Nanotoxicology ROS Dewey 333.7-333.9 Dewey 550 Swiss National Science Foundation Metal Bioavailability to Aquatic Photosynthetic Organisms in Changing Environment (BioChEn) 134627 CH Project funding 18 Institut des sciences de l'environnement 0DB 41 Département F.-A. Forel des sciences de l'environnement et de l'eau 153 false 752 Environmental Biogeochemistry and Ecotoxicology Aquatic toxicology 170 e6637873-e653-4f40-8200-54e42235667b Article (Published version) Restricted CuO plus lihgt pages 2016.pdf 2254595 application/pdf restrictedAccess Centres et instituts / Institut des sciences de l'environnement Faculté des sciences / Section des sciences de la Terre et de l'Environnement / Département F.-A. Forel des sciences de l'environnement et de l'eau

Title	Published in	Year	Views	Downloads

Interactive effects of copper oxide nanoparticles and light to green alga Chlamydomonas reinhardtii	Aquatic toxicology	2016	839	23
Combined exposure of Chlamydomonas reinhardtii to copper and solar-simulated radiation	SETAC Europe annual meeting	2013	443	0

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