Upregulation of IRAS/nischarin (I1-imidazoline receptor), a regulatory protein of μ-opioid receptor trafficking, in postmortem prefrontal cortex of long-term opiate and mixed opiate/cocaine abusers

Article Article scientifique Article Upregulation of IRAS/nischarin (I1-imidazoline receptor), a regulatory protein of μ-opioid receptor trafficking, in postmortem prefrontal cortex of long-term opiate and mixed opiate/cocaine abusers eng 2017 author Keller Benjamin 655555 author La Harpe Romano 125865 author García-Sevilla Jesús A 282-286 10.1016/j.neuint.2017.04.017 28461172 0197-0186 https://www.sciencedirect.com/science/article/pii/S019701861730178X Imidazoline receptor antisera-selected (IRAS)/nischarin, a putative I1-imidazoline receptor, has recently been shown to regulate μ-opioid receptor (OR) trafficking and resensitisation. To study a possible involvement of this μ-OR regulator in opiate dependence, the present study assessed by Western blot analysis the contents of IRAS/nischarin and μ-OR in total homogenates and subcellular preparations of postmortem human prefrontal cortex (PFC/BA9) of long-term opiate and mixed opiate/cocaine abusers as well as of matched healthy control subjects. In the PFC/BA9 of long-term opiate/cocaine abusers (all subjects together) IRAS/nischarin content was increased (+67%, p < 0.01, n = 11) when compared with matched controls (n = 10). Similar increases were found for the subgroups of opiate (+72%, n = 6) and mixed opiate/cocaine (+61%, n = 5) abusers. IRAS/nischarin immunocontents were also found increased in subcellular membrane preparations (+61%, p < 0.05, n = 10) of PFC/BA9 from opiate addicts. In the same brain samples, the levels of μ-OR were not different to those in control subjects. Based on the increased contents in brains of opiate abusers and the reported function as μ-OR regulator, IRAS/nischarin could represent a new promising target for treatment of opiate use disorder. Adult Cocaine-Related Disorders/metabolism/pathology Female Humans Imidazoline Receptors/biosynthesis Intracellular Signaling Peptides and Proteins/biosynthesis Male Middle Aged Opioid-Related Disorders/metabolism/pathology Prefrontal Cortex/metabolism/pathology Protein Transport/physiology Receptors Opioid Mu/metabolism Time Factors Up-Regulation/physiology IRAS/nischarin Μ-Opioid receptor Opiate dependence Imidazoline receptors Dewey 614.1 67 Institut de médecine légale 22A2 false 959 Groupe Silke Grabherr 969 Neurochemistry International 108 8630dc77-f75c-4f49-b634-4c4d62a48a5f Article (Published version) Restricted 28461172.pdf 677280 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de santé et médecine communautaires / Institut de médecine légale

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Upregulation of IRAS/nischarin (I1-imidazoline receptor), a regulatory protein of μ-opioid receptor trafficking, in postmortem prefrontal cortex of long-term opiate and mixed opiate/cocaine abusers	Neurochemistry International			2017	315	0

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Keller, Benjamin