SNX12 Role in Endosome Membrane Transport

Article Article scientifique Article SNX12 Role in Endosome Membrane Transport eng 2012 author Pons Véronique 584408 author Ustunel Eren Cansel 718930 author Rolland Corinne author Torti Eleonora 653484 author Parton Robert G. author Gruenberg Jean 29661 e38949 10.1371/journal.pone.0038949 1932-6203 http://dx.plos.org/10.1371/journal.pone.0038949 In this paper, we investigated the role of sorting nexin 12 (SNX12) in the endocytic pathway. SNX12 is a member of the PX domain-containing sorting nexin family and shares high homology with SNX3, which plays a central role in the formation of intralumenal vesicles within multivesicular endosomes. We found that SNX12 is expressed at very low levels compared to SNX3. SNX12 is primarily associated with early endosomes and this endosomal localization depends on the binding to 3-phosphoinositides. We find that overexpression of SNX12 prevents the detachment (or maturation) of multivesicular endosomes from early endosomes. This in turn inhibits the degradative pathway from early to late endosomes/lysosomes, much like SNX3 overexpression, without affecting endocytosis, recycling and retrograde transport. In addition, while previous studies showed that Hrs knockdown prevents EGF receptor sorting into multivesicular endosomes, we find that overexpression of SNX12 restores the sorting process in an Hrs knockdown background. Altogether, our data show that despite lower expression level, SNX12 shares redundant functions with SNX3 in the biogenesis of multivesicular endosomes. Dewey 540 39 Département de biochimie 144 false PloS one 7 6 9e26c1f1-b81e-46e3-8103-d0c7a79aa217 Article (Published version) Public journal.pone.0038949.pdf 899383 application/pdf CC BY openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie

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SNX12 Role in Endosome Membrane Transport	PloS one			2012	440	275

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Torti, Eleonora