Cytosolic Hsp90α and its mitochondrial isoform Trap1 are differentially required in a breast cancer model

Article Article scientifique Article Cytosolic Hsp90α and its mitochondrial isoform Trap1 are differentially required in a breast cancer model eng 2017 author Vartholomaiou Evangelia 752879 author Madon-Simon Marta 777365 author Hagmann Stéphane 642875 author Muhlebach Guillaume 523902 author Wurst Wolfgang author Floss Thomas author Picard Didier 0000-0001-8816-9668 26830 17428-17442 10.18632/oncotarget.15659 1949-2553 http://www.oncotarget.com/abstract/15659 The Hsp90 family of molecular chaperones includes the cytosolic isoforms Hsp90α and Hsp90β, and the mitochondrial isoform Trap1. Hsp90α/β support a large number of client proteins in the cytoplasm and the nucleus whereas Trap1 regulates oxidative phosphorylation in mitochondria. Many of the associated proteins and cellular processes are relevant to cancer, and there is ample pharmacological and genetic evidence to support the idea that Hsp90α/β and Trap1 are required for tumorigenesis. However, a direct and comparative genetic test in a mouse cancer model has not been done. Here we report the effects of deleting the Hsp90α or Trap1 genes in a mouse model of breast cancer. Neither Hsp90α nor Trap1 are absolutely required for mammary tumor initiation, growth and metastasis induced by the polyoma middle T-antigen as oncogene. However, they do modulate growth and lung metastasis in vivo and cell proliferation, migration and invasion of isolated primary carcinoma cells in vitro. Without Hsp90α, tumor burden and metastasis are reduced, correlating with impaired proliferation, migration and invasion of cells in culture. Without Trap1, the appearance of tumors is initially delayed, and isolated cells are affected similarly to those without Hsp90α. Analysis of expression data of human breast cancers supports the conclusion that this is a valid mouse model highlighting the importance of these molecular chaperones. Dewey 570 46 Département de biologie cellulaire 165 false 838 Groupe Picard Oncotarget 8 d45686a2-ba84-43b8-9f85-baa7fb92cbef Article (Published version) Public 15659-231019-3-PB.pdf 7064029 application/pdf openAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire

Title	Published in	Year	Views	Downloads

Cytosolic Hsp90α and its mitochondrial isoform Trap1 are differentially required in a breast cancer model	Oncotarget	2017	525	264
Mapping and characterization of the "enhancer of mom 6" mutant		2013	296	11

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Hagmann, Stéphane