Fluoroscopic Analysis of Protein Aggregates

Diplôme Master Fluoroscopic Analysis of Protein Aggregates eng 2011 author Al Sweidi Majed 642258 director Borchard Gerrit 0000-0001-6268-2586 635281 director Reichert Christian 739094 In the last decades protein drug formulations have become a field of emerging interest. Unfortunately, proteins have the tendency to form aggregates and lose their bioactivity in the process. In some cases the formation of aggregates has also been associated with toxicity. Therefore techniques and strategies to a) prevent protein formulations from aggregation and b) analyze formulations in order to recognize aggregation are essential. A known technique to analyze protein aggregation is fluorescence spectroscopy. Aggregated proteins often show a different fluoroscopic spectrum than in their native state. Furthermore, the use of extrinsic fluorescent dyes can reveal additional information about the Protein structure. We propose here a project in which the student will be working with a commercially available protein (e.g., lysozyme) and apply different stressors like heat, pH, ionic strength, to it, which are known to cause proteins to aggregate. He will also use trp-PEG in order to examine if it is possible to prevent lysozyme from aggregation. Afterwards he would analyze the proteins with fluorometric methods and determine the extent and nature of the aggregation. PARTIE EXPERIMENTALE Protein aggregation Fluorometrie Extrinsic Fluorescent Dyes Dewey 615 Maitrise universitaire en pharmacie 31 Section des sciences pharmaceutiques 17 false 48c3ebca-a0dc-4e8f-ae29-25516ad28861 Master thesis Restricted TPR_Al Sweidi_Majed.pdf 1595811 application/pdf f60ae261-5bc0-48d2-a80a-c47b3f001c21 Master thesis Restricted TPR_Annex_Majed Al Sweidi.pdf 1574809 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Al Sweidi, Majed