Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats

Article Article scientifique Article Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats eng 2012 author Vallée Jean-Paul 0000-0001-7546-2013 29185 author Hauwel Mathieu 629498 author Lepetit Coiffe Matthieu 640473 author Bei Wang author Montet-Abou Karin 94106 author Meda Paolo 26346 author Gardier Stéphany 684854 author Zammaretti Prisca author Kraehenbuehl Thomas P. author Herrmann François 70980 author Hubbell Jeffrey A. author Jaconi Marisa 248-260 10.5966/sctm.2011-0028 23197784 2157-6564 Pluripotent stem cell-seeded cardiopatches hold promise for in situ regeneration of infarcted hearts. Here, we describe a novel cardiopatch based on bone morphogenetic protein 2-primed cardiac-committed mouse embryonic stem cells, embedded into biodegradable fibrin matrices and engrafted onto infarcted rat hearts. For in vivo tracking of the engrafted cardiac-committed cells, superparamagnetic iron oxide nanoparticles were magnetofected into the cells, thus enabling detection and functional evaluation by high-resolution magnetic resonance imaging. Six weeks after transplantation into infarcted rat hearts, both local (p < .04) and global (p < .015) heart function, as well as the left ventricular dilation (p < .0011), were significantly improved (p < .001) as compared with hearts receiving cardiopatches loaded with iron nanoparticles alone. Histological analysis revealed that the fibrin scaffolds had degraded over time and clusters of myocyte enhancer factor 2-positive cardiac-committed cells had colonized most of the infarcted myocardium, including the fibrotic area. De novo CD31-positive blood vessels were formed in the vicinity of the transplanted cardiopatch. Altogether, our data provide evidence that stem cell-based cardiopatches represent a promising therapeutic strategy to achieve efficient cell implantation and improved global and regional cardiac function after myocardial infarction. Dewey 612 Dewey 616.07 Dewey 616.0757 Dewey 618.97 134 Département de physiologie cellulaire et métabolisme 21C 137 Département de pathologie et immunologie 21F 62 Département de radiologie et informatique médicale 227 65 Département de réadaptation et gériatrie 22B false 286 Imagerie cardiaque fonctionnelle 541 Stem cells translational medicine 1 3 a9ad5f96-8565-4ec1-98b4-59686d458ffe Article Restricted file1.pdf 2738087 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de physiologie cellulaire et métabolisme Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de radiologie et informatique médicale Faculté de médecine / Section de médecine clinique / Département de réadaptation et gériatrie

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Embryonic stem cell-based cardiopatches improve cardiac function in infarcted rats	Stem cells translational medicine			2012	716	8

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Lepetit Coiffe, Matthieu