The nuclear pore regulates GAL1 gene transcription by controlling the localization of the SUMO protease Ulp1

Article Article scientifique Article The nuclear pore regulates GAL1 gene transcription by controlling the localization of the SUMO protease Ulp1 eng 2013 author Texari Lorane 712466 author Dieppois Guennaelle 592919 author Vinciguerra Patrizia 135099 author Pardo Contreras Mariana 669044 author Groner Anna Claire 637681 author Letourneau Audrey 653025 author Stutz Françoise 64881 807-818 10.1016/j.molcel.2013.08.047 24074957 1097-2765 Transcription activation of some yeast genes correlates with their repositioning to the nuclear pore complex (NPC). The NPC-bound Mlp1 and Mlp2 proteins have been shown to associate with the GAL1 gene promoter and to maintain Ulp1, a key SUMO protease, at the NPC. Here, we show that the release of Ulp1 from the NPC increases the kinetics of GAL1 derepression, whereas artificial NPC anchoring of Ulp1 in the Δmlp1/2 strain restores normal GAL1 regulation. Moreover, artificial tethering of the Ulp1 catalytic domain to the GAL1 locus enhances the derepression kinetics. Our results also indicate that Ulp1 modulates the sumoylation state of Tup1 and Ssn6, two regulators of glucose-repressed genes, and that a loss of Ssn6 sumoylation correlates with an increase in GAL1 derepression kinetics. Altogether, our data highlight a role for the NPC-associated SUMO protease Ulp1 in regulating the sumoylation of gene-bound transcription regulators, positively affecting transcription kinetics in the context of the NPC. Cysteine Endopeptidases/genetics Galactokinase/genetics/metabolism Nuclear Pore/genetics/metabolism Nuclear Proteins/metabolism Promoter Regions, Genetic Repressor Proteins/metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins/genetics/metabolism Sumoylation Transcription, Genetic Transcriptional Activation Dewey 570 Dewey 576.5 Swiss National Science Foundation Epigenetic control of gene expression by antisense RNA and functional coupling of mRNA biogenesis and export through nuclear pores in S. cerevisiae 130292 CH Project funding 46 Département de biologie cellulaire 165 135 Département de médecine génétique et développement 21D false 839 Groupe Stutz Molecular cell 51 6 52f9b0e9-5fd0-461f-9101-d1576a332689 Article (Published version) Restricted Texari2.pdf 3321071 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département de biologie cellulaire Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement

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The nuclear pore regulates GAL1 gene transcription by controlling the localization of the SUMO protease Ulp1	Molecular cell			2013	694	1

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Groner, Anna Claire