Blue light-mediated inactivation of <i>Enterococcus faecalis</i> in vitro

Article Article scientifique Article Blue light-mediated inactivation of <i>Enterococcus faecalis</i> in vitro eng 2013 author Pileggi Giorgio 632946 author Wataha John C. author Girard Myriam 666631 author Lange Norbert 135650 author Schrenzel Jacques 29184 author Grad Iwona 585158 author Bouillaguet Serge 23861 134-140 10.1016/j.pdpdt.2012.11.002 23769279 1572-1000 https://www.sciencedirect.com/science/article/pii/S157210001200107X In dentistry, residual infection remains a major cause of failure after endodontic treatment; many of these infections involve Enterococcus faecalis. In the current study, we explored the possibility that blue light activated photosensitizers could be used, in principle, to inactivate this microbe as an adjunct disinfection strategy for endodontic therapy. Three blue light absorbing photosensitizers, eosin-Y, rose bengal, and curcumin, were tested on E. faecalis grown in planktonic suspensions or biofilms. Photosensitizers were incubated for 30 min with bacteria then exposed to blue light (450-500 nm) for 240 s. Sodium hypochlorite (3%) was used as a control. After 48 h, the viability of E. faecalis was estimated by measuring colony-forming units post-exposure vs. untreated controls (CFU/mL). Blue light irradiation alone did not alter E. faecalis viability. For planktonic cultures, blue light activated eosin-Y (5 μM), rose bengal (1 μM), or curcumin (5 μM) significantly (p<0.05) reduced E. faecalis viability compared to exposure to the unirradiated photochemicals. For biofilm cultures, concentrations of light-activated eosin-Y, rose bengal, and curcumin of 100, 10, and 10 μM respectively, completely suppressed E. faecalis viability (p<0.05). Although the current results are limited to an in vitro model, they support further exploration of blue light activated antimicrobials as an adjunct therapy in endodontic treatment. Biofilm Blue light Endodontic infection Enterococcus faecalis PACT Apoptosis / drug effects Apoptosis / radiation effects Cell Survival / drug effects Cell Survival / radiation effects Color Curcumin / administration & dosage Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Enterococcus faecalis / cytology Enterococcus faecalis / drug effects Enterococcus faecalis / radiation effects Eosine Yellowish-(YS) / administration & dosage Fluorescent Dyes / administration & dosage Lighting / methods Microbial Viability / drug effects Microbial Viability / radiation effects Photochemotherapy / methods Photosensitizing Agents / administration & dosage Rose Bengal / administration & dosage Dewey 617.6 Dewey 616 Swiss National Science Foundation Blue light-mediated inactivation of endodontic pathogens 119938 CH Projects 56 Département de médecine 221 205 Division de cariologie et d'endodontie 24P1 false 338 Génomique et microbiote 604 508 Groupe Bouillaguet Serge (medecine dentaire) 846 Photodiagnosis and photodynamic therapy 10 2 320eba58-4943-458a-85c2-0b8a7917fc34 Article (Published version) Restricted main.pdf 790051 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine dentaire / Département de médecine dentaire préventive et de premier recours / Division de cariologie et d'endodontie

Title	Published in	Year	Views	Downloads

Inactivation d'Enterococcus Faecalis à l'aide d'agents photo-activables à la lumière bleue		2013	784	2
Blue light-mediated inactivation of Enterococcus faecalis in vitro	Photodiagnosis and photodynamic therapy	2013	683	9

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