Bioaccumulation and molecular effects of carbamazepine and methylmercury co-exposure in males of Dreissena polymorpha

Article Article scientifique Article Bioaccumulation and molecular effects of carbamazepine and methylmercury co-exposure in males of Dreissena polymorpha eng 2023-11 author Baratange Clément 926848 author Baali Hugo author Gaillet Véronique author Bonnard Isabelle author Delahaut Laurence author Gaillard Jean-Charles author Grandjean Dominique author Sayen Stéphanie author Gallorini Andrea 876623 author Le Bris Nathalie author Renault David author Breider Florian 632890 author Loizeau Jean-Luc 0000-0002-0611-0388 23020 Forel Communs (FOREL) author Armengaud Jean author Cosio Claudia 89306 1-13 165379 10.1016/j.scitotenv.2023.165379 37423277 0048-9697 https://linkinghub.elsevier.com/retrieve/pii/S0048969723040020 Dreissena polymorpha is a bivalve promising for biomonitoring in freshwater ecosystems thanks to its abundance and high filtration activity allowing rapid uptake of toxicants and identification of their negative effects. Nonetheless, we still lack knowledge on its molecular responses to stress under realistic scenario, e.g. multi-contamination. Carbamazepine (CBZ) and Hg are ubiquitous pollutants sharing molecular toxicity pathways, e.g. oxidative stress. A previous study in zebra mussels showed their co-exposure to cause more alterations than single exposures, but molecular toxicity pathways remained unidentified. D. polymorpha was exposed 24 h (T24) and 72 h (T72) to CBZ (6.1±0.1 μg L−1), MeHg (430±10 ng L−1) andthe coexposure (6.1 ± 0.1 μg L−1CBZ and 500 ± 10 ng L−1 MeHg) at concentrations representative of polluted areas (~10× EQS). RedOx system at the gene and enzyme level, the proteome and the metabolome were compared. The co-exposure resulted in 108 differential abundant proteins (DAPs), as well as 9 and 10 modulated metabolites at T24 and T72, respectively. The co-exposure specifically modulated DAPs and metabolites involved in neurotransmission, e.g. dopaminergic synapse and GABA. CBZ specifically modulated 46 DAPs involved in calcium signaling pathways and 7 amino acids at T24. MeHg specifically modulated 55 DAPs involved in the cytoskeleton remodeling and hypoxia-induced factor 1 pathway, without altering the metabolome. Single and co-exposures commonly modulated proteins and metabolites involved in energy and amino acid metabolisms, response to stress and development. Concomitantly, lipid peroxidation and antioxidant activities were unchanged, supporting that D. polymorpha tolerated experimental conditions. The co-exposure was confirmed to cause more alterations than single exposures. This was attributed to the combined toxicity of CBZ and MeHg. Altogether, this study underlined the necessity to better characterize molecular toxicity pathways ofmulti-contamination that are not predictable on responses to single exposures, to better anticipate adverse effects in biota and improve risk assessment. Bivalve Freshwater Multi-omics Toxicity pathways Dewey 550 Dewey 333.7-333.9 41 Département F.-A. Forel des sciences de l'environnement et de l'eau 153 18 Institut des sciences de l'environnement 0DB false 753 Limnology and Environmental Geology 938 ISE Pôle Sciences 944 ISE Eau Science of the total environment 897 acccdf36-c7e5-4a70-9f0f-2bccb30ddd56 Article (Published version) Restricted Barratange et al 2023 STOTEN.pdf 3133572 application/pdf 17f641f0e92cecedf1c0a51b0932849f21922f2af6968d193302c9d62d079ab8 restrictedAccess Faculté des sciences / Section des sciences de la Terre et de l'Environnement / Département F.-A. Forel des sciences de l'environnement et de l'eau Centres et instituts / Institut des sciences de l'environnement

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Bioaccumulation and molecular effects of carbamazepine and methylmercury co-exposure in males of Dreissena polymorpha	Science of the total environment			2023	87	0

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