The CshA DEAD-box RNA helicase is important for quorum sensing control in Staphylococcus aureus

Article Article scientifique Article The CshA DEAD-box RNA helicase is important for quorum sensing control in Staphylococcus aureus eng 2013 author Oun Stella 630023 author Redder Peter 689479 author Didier Jean-Philippe 713912 author Francois Patrice 0000-0002-0540-750X 28911 author Corvaglia Anna-Rita author Buttazzoni Elena 667695 author Giraud Caroline 762836 author Girard Myriam 666631 author Schrenzel Jacques 29184 author Linder Patrick 61214 157-165 10.4161/rna.22899 23229022 1547-6286 DEAD-box RNA helicases are present in almost all living organisms and participate in various processes of RNA metabolism. Bacterial proteins of this large family were shown to be required for translation initiation, ribosome biogenesis and RNA decay. The latter is primordial for rapid adaptation to changing environmental conditions. In particular, the RhlB RNA helicase from E. coli was shown to assist the bacterial degradosome machinery. Recently, the CshA DEAD-box proteins from Bacillus subtilis and Staphylococcus aureus were shown to interact with proteins that are believed to form the degradosome. S. aureus can cause life-threatening disease, with particular concern focusing on biofilm formation on catheters and prosthetic devices, since in this form the bacteria are almost impossible to eradicate both by the immune system and antibiotic treatment. This persistent state relies on the expression of surface encoded proteins that allow attachment to various surfaces, and contrasts with the dispersal mode of growth that relies on the secretion of proteins such as hemolysins and proteases. The switch between these two states is mainly mediated by the Staphylococcal cell density sensing system encoded by agr. We show that inactivation of the cshA DEAD-box gene results in dysregulation of biofilm formation and hemolysis through modulation of agr mRNA stability. Importantly, inactivation of the agrA gene in the cshA mutant background reverses the defect, indicating that cshA is genetically upstream of agr and that a delicate balance of agr mRNA abundance mediated through stability control by CshA is critical for proper expression of virulence factors. Dewey 616 54 Département de microbiologie et médecine moléculaire 21B false 63 Acquisition et expression de facteurs de virulence chez Staphylococcus aureus 86 338 Génomique et microbiote 604 RNA biology 10 1 21c587e8-8ae6-431b-a04c-653c41399e13 Article (Published version) Public rna-10-157.pdf 687072 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

Title	Published in	Year	Views	Downloads

The CshA DEAD-box RNA helicase is important for quorum sensing control in Staphylococcus aureus	RNA biology	2013	755	557
Characterization of the DEAD-Box RNA Helicase CshA from Staphylococcus aureus		2012	941	744

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