Melatonin improves muscle function of the dystrophic mdx(5Cv) mouse, a model for Duchenne muscular dystrophy

Article Article scientifique Article Melatonin improves muscle function of the dystrophic mdx(5Cv) mouse, a model for Duchenne muscular dystrophy eng 2011 author Hibaoui Youssef 623159 author Patte Julie 624977 author Patthey Ophelie 135760 author Ruegg Urs 135655 author Dorchies Olivier 135677 163-171 10.1111/j.1600-079X.2011.00871.x 21486366 1600-079X Duchenne muscular dystrophy (DMD) is a severe X-linked muscle-wasting disease caused by the absence of the cytoskeletal protein dystrophin. In addition to abnormal calcium handling, numerous studies point to a crucial role of oxidative stress in the pathogenesis of the disease. Considering the impressive results provided by antioxidants on dystrophic muscle structure and function, we investigated whether melatonin can protect the mdx(5Cv) mouse, an animal model for DMD. Male mdx(5Cv) mouse pups were treated with melatonin by daily intraperitoneal (i.p.) injection (30 mg/kg body weight) or by subcutaneous (s.c.) implant(s) (18 or 54 mg melatonin as Melovine(®) implants) from 17/18 to 28/29 days of age. Isometric force of the triceps surae was recorded at the end of the treatment. The i.p. treatment increased the phasic twitch tension of mdx(5Cv) mice. The maximal tetanic tension was ameliorated by 18 mg s.c. and 30 mg/kg i.p. treatments. Melatonin caused the dystrophic muscle to contract and relax faster. The force-frequency relationship of melatonin-treated dystrophic mice was shifted to the right. In accordance with improved muscle function, melatonin decreased plasma creatine kinase activity, a marker for muscle injury. Melatonin treatment increased total glutathione content and lowered the oxidized/reduced glutathione ratio, indicating a better redox status of the muscle. In light of the present investigation, the therapeutic potential of melatonin should be further considered for patients with DMD. Dewey 615 31 Section des sciences pharmaceutiques 17 false Journal of pineal research 51 2 2e374024-4228-4084-b6d5-06467c99b3e6 Article (Published version) Restricted Melatonin improves muscle function of the dystrophic mdx5cv mouse, a model for Duchenne meuscular dystrophy.pdf 338670 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

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Melatonin improves muscle function of the dystrophic mdx(5Cv) mouse, a model for Duchenne muscular dystrophy	Journal of pineal research			2011	645	5

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Patte, Julie