Tropane Alkaloids in Transformed Roots of <i>Datura quercifolia</i>

Article Article scientifique Article Tropane Alkaloids in Transformed Roots of <i>Datura quercifolia</i> eng 2007-01-04 2007-01-04 author Dupraz Jean-Marc 62213 author Christen Philippe 23100 author Kapetanidis Ilias 24938 158-162 10.1055/s-2006-959441 17236033 0032-0943 Hairy root cultures of Datura quercifolia were established following infection with Agrobacterium rhizogenes strain LBA 9402. Eight tropane alkaloids were identified in the hairy roots, hyoscyamine being the major comtituent. The growth and the hyoscyamine content of transformed roots were investigated under various conditions. Gamborg B5, Mun,.shige and Skoog, and Woody Plant media were tested. Gamborg B5 medium was the best for growth as well as for hyoscyamine accumulation. The influence of sm;rose concentration was examined and a 5 % concentration was found to be the most appropriate for growth and for alkaloid production. After 35 days of incubation in this medium, the hyoscyamine content of the roots was 1. 24 % based on dry weight. The influence of gibberellic acid and of Amberlite XAD-4 resin on hyoscyamine production was tested. Datura quercifolia Solanaceae. Agrobacterium rhizogenes Tropane alkaloids GC-MS HPLC Sucrose Gibberellic add Amberlite XAD-4 resin Dewey 615 31 Section des sciences pharmaceutiques 17 false Planta medica 60 02 1244ffa5-8cf6-4888-a213-e0c42ba07202 Article (Published version) Restricted Dupraz_PM_199460158.pdf 3386028 application/pdf 5cd03331162673b61da0176f600c87119183a21d5ab5dcec8594e4dce0ca47e3 restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

Title	Published in	Access level	OA Policy	Year	Views	Downloads

Tropane Alkaloids in Transformed Roots of Datura quercifolia	Planta medica			2007	71	1

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Dupraz, Jean-Marc