Sendai virus RNA polymerase scanning for mRNA start sites at gene junctions

Article Article scientifique Article Sendai virus RNA polymerase scanning for mRNA start sites at gene junctions eng 2007 author Plattet Philippe 621941 author Strahle Laura Eve 504345 author Le Mercier Philippe 514147 author Hausmann Stéphane 29303 author Garcin Dominique 24296 author Kolakofsky Daniel 25420 411-420 10.1016/j.virol.2006.12.033 17300823 0042-6822 Mini-genomes expressing two reporter genes and a variable gene junction were used to study Sendai virus RNA polymerase (RdRp) scanning for the mRNA start signal of the downstream gene (gs2). We found that RdRp could scan the template efficiently as long as the initiating uridylate of gs2 (3' UCCCnnUUUC) was preceded by the conserved intergenic region (3' GAA) and the last 3 uridylates of the upstream gene end signal (ge1; 3' AUUCUUUUU). The end of the leader sequence (3' CUAAAA, which precedes gs1) could also be used for gene2 expression, but this sequence was considerably less efficient. Increasing the distance between ge1 and gs2 (up to 200 nt) led to the progressive loss of gene2 expression, in which half of gene2 expression was lost for each 70 nucleotides of intervening sequence. Beyond 200 nt, gene2 expression was lost more slowly. Our results suggest that there may be two populations of RdRp that scan at gene junctions, which can be distinguished by the efficiency with which they can scan the genome template for gs. Animals Base Sequence Cell Line Conserved Sequence Cricetinae DNA, Intergenic/genetics DNA-Directed RNA Polymerases/metabolism Genes, Reporter/genetics Green Fluorescent Proteins/biosynthesis/genetics Luminescent Proteins/biosynthesis/genetics Molecular Sequence Data RNA, Messenger/biosynthesis/genetics RNA, Viral/biosynthesis/genetics Sendai virus/enzymology Transcription, Genetic Dewey 616 54 Département de microbiologie et médecine moléculaire 21B false 216 Virologie moléculaire 275 Virology 362 2 7909df7a-40a1-4bac-9242-2cc693b9274c Article (Published version) Public 1-s2.0-S004268220600938X-main.pdf 1042846 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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Sendai virus RNA polymerase scanning for mRNA start sites at gene junctions	Virology			2007	549	358

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Plattet, Philippe