The role of chromatin-remodeling factor PKL in balancing osmotic stress responses during Arabidopsis seed germination

Article Article scientifique Article The role of chromatin-remodeling factor PKL in balancing osmotic stress responses during Arabidopsis seed germination eng 2007 author Perruc Elian 604397 author Kinoshita Natsuko 598341 author Lopez Molina Luis 0000-0003-0463-1187 28870 927-936 10.1111/j.1365-313X.2007.03288.x 17892443 0960-7412 Embryogenesis in Arabidopsis results in mature, osmotolerant embryos within dry seeds. Late-embryogenesis programs involve the transcription factors ABI3 and ABI5, which are necessary for osmotolerance. ABI3 and ABI5 are degraded in seeds initiating germination, abolishing their protected state. However, during an early stage of germination, strong osmotic stresses, or ABA exposure maintain ABI3 and ABI5 expression, leading to growth arrest, and osmotolerance. Mild stress stimuli delay ABI3 and ABI5 disappearance, retarding germination but not preventing eventual closure of embryogenesis programs. PICKLE (PKL), a putative chromatin modifier, is necessary to repress ABI3 and ABI5 expression during germination in response to ABA. We show that pkl mutants display persistent high expression of ABI3 and ABI5 upon ABA stimulation. In turn, maintenance of ABI5 expression leads to hypersensitive germination responses to ABA in pkl seeds. We provide evidence that ABI3 and ABI5 are less associated with repressed chromatin in pkl mutants. Our results provide evidence that PKL-dependent repression of embryonic gene expression extends to late-embryogenesis genes and is associated with changes in chromatin. We suggest that effective closure of embryogenesis omostolerance programs during germination prevents excessive plant reactions to stress. Arabidopsis/embryology/genetics/metabolism Arabidopsis Proteins/genetics/metabolism/physiology Basic-Leucine Zipper Transcription Factors/genetics/metabolism Chromatin Assembly and Disassembly DNA Helicases Gene Expression Regulation, Plant Germination Osmotic Pressure Seeds/genetics/growth & development/metabolism Transcription Factors Dewey 580 45 Département des sciences végétales 163 false 831 Groupe Lopez-Molina Plant journal 52 5 8698f5d1-b8ee-4bcb-bbbd-38f6f766a3ba Article (Published version) Restricted Perruc et al 2007.pdf 231131 application/pdf 49759c3c-1eb5-43e4-8b6a-921e2b057143 Article (Published version) Restricted supmat_figs.pdf 5338098 application/pdf restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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The role of chromatin-remodeling factor PKL in balancing osmotic stress responses during Arabidopsis seed germination	Plant journal			2007	592	0

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Perruc, Elian