Gram-negative quorum sensing signalling enhances biofilm formation and virulence traits in gram-positive pathogen Enterococcus faecalis

Article Article scientifique Article Gram-negative quorum sensing signalling enhances biofilm formation and virulence traits in gram-positive pathogen Enterococcus faecalis eng 2023-05-11 2023 author Parga Martinez Ana 0000-0002-7670-6603 Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.,Division of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden. 408513 CUMD - Biomatériaux (BMAT) author Manoil Daniel 0000-0002-9220-3977 Division of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.,Division of cariology and endodontics, University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland. 660428 CUMD - Clinique de cariologie et d'endodontie (CCAR) author Brundin Malin Division of Endodontics, Department of Odontology, Umeå University, Umeå, Sweden. author Otero Ana Department of Microbiology and Parasitology, CIBUS-Faculty of Biology, Universidade de Santiago de Compostela, Santiago de Compostela, Spain. 600117 author Belibasakis Georgios N Division of Oral Diseases, Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden. 2208901 10.1080/20002297.2023.2208901 37187675 2000-2297 PMC10177678 Acyl-homoserine lactones (AHLs) are typical quorum-sensing molecules of gram-negative bacteria. Recent evidence suggests that AHLs may also affect gram-positives, although knowledge of these interactions remains scarce. Here, we assessed the effect of AHLs on biofilm formation and transcriptional regulations in the gram-positiveEnterococcus faecalis. FiveE. faecalisstrains were investigated herein. Crystal violet was employed to quantify the biomass formed, and confocal microscopy in combination with SYTO9/PI allowed the visualisation of biofilms' structure. The differential expression of 10 genes involved in quorum-sensing, biofilm formation and stress responses was evaluated using reverse-transcription-qPCR. The AHL exposure significantly increased biofilm production in strain ATCC 29212 and two isolates from infected dental roots, UmID4 and UmID5. In strains ATCC 29212 and UmID7, AHLs up-regulated the quorum-sensing genes (fsrC,cylA), the adhesinsace,efaAandasa1, together with the glycosyltransferaseepaQ. In strain UmID7, AHL exposure additionally up-regulated two membrane-stress response genes (σV,groEL) associated with increased stress-tolerance and virulence. Altogether, our results demonstrate that AHLs promote biofilm formation and up-regulate a transcriptional network involved in virulence and stress tolerance in severalE. faecalisstrains. These data provide yet-unreported insights intoE. faecalisbiofilm responses to AHLs, a family of molecules long-considered the monopole of gram-negative signalling. Enterococcus faecalis Fsrc Acyl-homoserine lactones Biofilm formation Cell-to-cell communication Endodontic infection Gene expression Quorum sensing Reverse-transcription quantitative PCR Virulence Dewey 617.6 205 Division de cariologie et d'endodontie 24P1 false 189 Groupe Krejci Ivo (médecine dentaire) 240 Journal of oral microbiology 15 1 87fecfd6-f7cd-4d04-9af9-450d2bac0a00 Article (Published version) Public Article-scientifique-from-europePmc.pdf 4647149 application/pdf CC BY-4.0 e4fdddecb1281164792d198e4b10cef591db79bb5df220f82a46ca12b1d382df openAccess Faculté de médecine / Section de médecine dentaire / Département de médecine dentaire préventive et de premier recours / Division de cariologie et d'endodontie

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Gram-negative quorum sensing signalling enhances biofilm formation and virulence traits in gram-positive pathogen Enterococcus faecalis	Journal of oral microbiology			2023	102	71

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