NopP, a phosphorylated effector of Rhizobium sp. strain NGR234, is a major determinant of nodulation of the tropical legumes Flemingia congesta and Tephrosia vogelii

Article Article scientifique Article NopP, a phosphorylated effector of Rhizobium sp. strain NGR234, is a major determinant of nodulation of the tropical legumes Flemingia congesta and Tephrosia vogelii eng 2005-07-20 2005-09-20 author Skorpil Peter 503901 author Saad Maged 517004 author Boukli Hacene Tani Nawal Maria 98513 author Kobayashi Hajime 542748 author Ares Orpel Florencia 594053 Département des sciences végétales (BIVEG) author Broughton William John 22724 Administration de la faculté des sciences (SIAD) author Deakin William James 507597 1304-1317 10.1111/j.1365-2958.2005.04768.x 16102002 0950-382X Rhizobium sp. NGR234 nodulates many plants, some of which react to proteins secreted via a type three secretion system (T3SS) in a positive- (Flemingia congesta, Tephrosia vogelii) or negative- (Crotalaria juncea, Pachyrhizus tuberosus) manner. T3SSs are devices that Gram-negative bacteria use to inject effector proteins into the cytoplasm of eukaryotic cells. The only two rhizobial T3SS effector proteins characterized to date are NopL and NopP of NGR234. NopL can be phosphorylated by plant kinases and we show this to be true for NopP as well. Mutation of nopP leads to a dramatic reduction in nodule numbers on F. congesta and T. vogelii. Concomitant mutation of nopL and nopP further diminishes nodulation capacity to levels that, on T. vogelii, are lower than those produced by the T3SS null mutant NGRΩrhcN. We also show that the T3SS of NGR234 secretes at least one additional effector, which remains to be identified. In other words, NGR234 secretes a cocktail of effectors, some of which have positive effects on nodulation of certain plants while others are perceived negatively and block nodulation. NopL and NopP are two components of this mix that extend the ability of NGR234 to nodulate certain legumes. Dewey 580 45 Département des sciences végétales 163 false Molecular microbiology 57 5 819ff245-2311-40af-a084-415c4a530807 Article (Published version) Restricted Skorpil_MMB_2005571304.pdf 477272 application/pdf 9c2b9b651920032d80282fb4d8a45ad762deb5dace5e314180994b44636ab045 restrictedAccess Faculté des sciences / Section de biologie / Département des sciences végétales

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NopP, a phosphorylated effector of Rhizobium sp. strain NGR234, is a major determinant of nodulation of the tropical legumes Flemingia congesta and Tephrosia vogelii	Molecular microbiology			2005	65	0

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