New insights into myosin evolution and classification

Article Article scientifique Article New insights into myosin evolution and classification eng 2006 author Foth Bernardo Javier 619237 author Goedecke Marc Christian 591141 author Soldati-Favre Dominique 0000-0003-4156-2109 135114 3681-3686 10.1073/pnas.0506307103 16505385 0027-8424 Myosins are eukaryotic actin-dependent molecular motors important for a broad range of functions like muscle contraction, vision, hearing, cell motility, and host cell invasion of apicomplexan parasites. Myosin heavy chains consist of distinct head, neck, and tail domains and have previously been categorized into 18 different classes based on phylogenetic analysis of their conserved heads. Here we describe a comprehensive phylogenetic examination of many previously unclassified myosins, with particular emphasis on sequences from apicomplexan and other chromalveolate protists including the model organism Toxoplasma, the malaria parasite Plasmodium, and the ciliate Tetrahymena. Using different phylogenetic inference methods and taking protein domain architectures, specific amino acid polymorphisms, and organismal distribution into account, we demonstrate a hitherto unrecognized common origin for ciliate and apicomplexan class XIV myosins. Our data also suggest common origins for some apicomplexan myosins and class VI, for classes II and XVIII, for classes XII and XV, and for some microsporidian myosins and class V, thereby reconciling evolutionary history and myosin structure in several cases and corroborating the common coevolution of myosin head, neck, and tail domains. Six novel myosin classes are established to accommodate sequences from chordate metazoans (class XIX), insects (class XX), kinetoplastids (class XXI), and apicomplexans and diatom algae (classes XXII, XXIII, and XXIV). These myosin (sub)classes include sequences with protein domains (FYVE, WW, UBA, ATS1-like, and WD40) previously unknown to be associated with myosin motors. Regarding the apicomplexan "myosome," we significantly update class XIV classification, propose a systematic naming convention, and discuss possible functions in these parasites. Animals Apicomplexa/chemistry/genetics Chordata Ciliophora/chemistry/genetics Evolution, Molecular Insects/chemistry/genetics Kinetoplastida/chemistry/genetics Microsporidia/chemistry/genetics Models, Genetic Molecular Sequence Data Myosins/chemistry/classification/genetics Phylogeny Plasmodium/chemistry/genetics Dewey 616 Swiss National Science Foundation Dissection of gliding motility and host cell invasion by the apicomplexan parasites CH Project funding 54 Département de microbiologie et médecine moléculaire 21B false 443 Biologie d'un parasite intracellulaire obligatoire 773 Proceedings of the National Academy of Sciences of the United States of America 103 10 b8456d4f-5a95-416c-ba14-b612e82b4b8a Article (Accepted version) Public PNAS-2006-Foth-3681-6.pdf 2053350 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire

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New insights into myosin evolution and classification	Proceedings of the National Academy of Sciences of the United States of America			2006	645	456

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Goedecke, Marc Christian