Autologous transplantation of porcine myogenic precursor cells in skeletal muscle

Article Article scientifique Article Autologous transplantation of porcine myogenic precursor cells in skeletal muscle eng 2005 author Holzer Nicolas 0000-0002-4855-8420 105042 author Hogendoorn Simone 590757 author Zurcher Line 599578 author Garavaglia Guido 590317 author Yang Sheng 560941 author Konig Stéphane 0000-0002-0801-279X 133957 author Laumonier Thomas 0000-0002-8662-2972 135589 author Menetrey Jacques 105033 237-244 10.1016/j.nmd.2004.11.001 15725585 0960-8966 Myoblast transplantation is a potential therapy for severe muscle trauma, myopathies and heart infarct. Success with this therapy relies on the ability to obtain cell preparations enriched in myogenic precursor cells and on their survival after transplantation. To define myoblast transplantation strategies applicable to patients, we used a large animal model, the pig. Muscle dissociation procedures adapted to porcine tissue gave high yields of cells containing at least 80% myogenic precursor cells. Autologous transplantation of 3[H]-thymidine labeled porcine myogenic precursor cells indicated 60% survival at day 1 followed by a decay to 10% at day 5 post-injection. Nuclei of myogenic precursor cells transduced with a lentivirus encoding the nls-lacZ reporter gene were present in host myotubes 8 days post-transplantation, indicating that injected myogenic precursor cells contribute to muscle regeneration. This work suggests that pig is an adequate large animal model for exploring myogenic precursor cells transplantation strategies applicable in patients. Animals Antigens, CD56/metabolism Cell Count/methods Cell Proliferation Cell Survival/physiology Desmin/metabolism Female Flow Cytometry Indoles/diagnostic use Muscle Development Muscle, Skeletal/physiology/transplantation Myoblasts/physiology/transplantation Swine Thymidine/metabolism Time Factors Transduction, Genetic Transplantation, Autologous Tritium/metabolism Dewey 616.8 Dewey 617 53 Département de neurosciences fondamentales 21A 57 Département de chirurgie 222 false 955 Thérapie cellulaire et troubles musculo-squelettiques 960 1464 Biomécanique et recherche translationnelle en chirurgie 1074 Neuromuscular disorders 15 3 6524da0c-c5f4-4d11-85ae-261b3789abd6 Article (Published version) Restricted 1-s2.0-S0960896604003128-main.pdf 430443 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de neurosciences fondamentales Faculté de médecine / Section de médecine clinique / Département de chirurgie

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Autologous transplantation of porcine myogenic precursor cells in skeletal muscle	Neuromuscular disorders			2005	878	0

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Hogendoorn, Simone