The Rivet Model for Channel Formation by Aerolysin-like Pore-Forming Toxins

Article Article scientifique Article The Rivet Model for Channel Formation by Aerolysin-like Pore-Forming Toxins eng 2006 author Iacovache Mircea Ioan 586762 author Paumard Patrick author Scheib Holger 594945 author Lesieur Claire 90717 author Sakai Naomi 0000-0002-9460-1944 131140 author Matile Stefan 0000-0002-8537-8349 129654 author Parker Michael W. author Van der Goot F. Gisou 457-466 10.1038/sj.emboj.7600959 16424900 0261-4189 The bacterial toxin aerolysin kills cells by forming heptameric channels, of unknown structure, in the plasma membrane. Using disulfide trapping and cysteine scanning mutagenesis coupled to thiol-specific labeling on lipid bilayers, we identify a loop that lines the channel. This loop has an alternating pattern of charged and uncharged residues, suggesting that the transmembrane region has a beta-barrel configuration, as observed for Staphylococcal alpha-toxin. Surprisingly, we found that the turn of the beta-hairpin is composed of a stretch of five hydrophobic residues. We show that this hydrophobic turn drives membrane insertion of the developing channel and propose that, once the lipid bilayer has been crossed, it folds back parallel to the plane of the membrane in a rivet-like fashion. This rivet-like conformation was modeled and sequence alignments suggest that such channel riveting may operate for many other pore-forming toxins. ß-barrel Lipid bilayers Membrane proteins Pore-forming Toxin Dewey 540 36 Département de chimie organique 141 false EMBO journal 25 3 711fa8f5-f975-42ec-b8ab-265ef8f72a5e Article Restricted file1.pdf 357319 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de chimie organique

Title	Published in	Access level	OA Policy	Year	Views	Downloads

The Rivet Model for Channel Formation by Aerolysin-like Pore-Forming Toxins	EMBO journal			2006	689	1

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Iacovache, Mircea Ioan