A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC)

Article Article scientifique Article A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC) eng 2016 author Alessandri Kevin author Feyeux Maxime 755543 author Gurchenkov Basile author Delgado Christophe 583419 author Trushko Anastasiya 798392 author Krause Karl-Heinz 23022 author Vignjević Daniela author Nassoy Pierre author Roux Aurélien 0000-0002-6088-0711 716946 1593-1604 10.1039/c6lc00133e 27025278 1473-0189 We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneous instability of a multi-layered jet formed by co-extrusion using a coaxial flow device. We provide a simple design to manufacture this device using a DLP (digital light processing) 3D printer. Then, we demonstrate how the inner wall of the capsules can be decorated with a continuous ECM layer that is anchored to the alginate gel and mimics the basal membrane of a cellular niche. Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. Altogether, we show that these capsules may serve as cell micro-containers compatible with complex cell culture conditions and applications. These developments widen the field of research and biomedical applications of the cell encapsulation technology. Dewey 540 Dewey 616.07 39 Département de biochimie 144 137 Département de pathologie et immunologie 21F false 38 Radicaux libres et cellules souches embryonnaires 60 Lab on a chip 16 9 2c8b4675-5204-4ff1-9368-bca8e555c29e Article (Published version) Public 27025278.pdf 3697146 application/pdf openAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC)	Lab on a chip			2016	1,018	1,634

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Delgado, Christophe