Silencing of c-Fos expression by microRNA-155 is critical for dendritic cell maturation and function

Article Article scientifique Article Silencing of c-Fos expression by microRNA-155 is critical for dendritic cell maturation and function eng 2011 author Dunand-Sauthier Isabelle 134225 author Santiago-Raber Marie-Laure 556511 author Capponi Léonardo Piero 564473 author Vejnar Charles E author Schaad Olivier 0000-0003-1576-783X 23118 author Irla Magali 638461 author Seguin Estévez Maria Queralt 585258 author Descombes Patrick 24132 author Zdobnov Evgeny M author Acha-Orbea Hans author Reith Walter 25482 4490-4500 10.1182/blood-2010-09-308064 21385848 0006-4971 MicroRNAs (miRNAs) are small, noncoding RNAs that regulate target mRNAs by binding to their 3' untranslated regions. There is growing evidence that microRNA-155 (miR155) modulates gene expression in various cell types of the immune system and is a prominent player in the regulation of innate and adaptive immune responses. To define the role of miR155 in dendritic cells (DCs) we performed a detailed analysis of its expression and function in human and mouse DCs. A strong increase in miR155 expression was found to be a general and evolutionarily conserved feature associated with the activation of DCs by diverse maturation stimuli in all DC subtypes tested. Analysis of miR155-deficient DCs demonstrated that miR155 induction is required for efficient DC maturation and is critical for the ability of DCs to promote antigen-specific T-cell activation. Expression-profiling studies performed with miR155(-/-) DCs and DCs overexpressing miR155, combined with functional assays, revealed that the mRNA encoding the transcription factor c-Fos is a direct target of miR155. Finally, all of the phenotypic and functional defects exhibited by miR155(-/-) DCs could be reproduced by deregulated c-Fos expression. These results indicate that silencing of c-Fos expression by miR155 is a conserved process that is required for DC maturation and function. Animals Cell Differentiation/genetics/immunology Cell Line Dendritic Cells/cytology/physiology Evolution, Molecular Gene Silencing/immunology Humans Mice Mice, Mutant Strains MicroRNAs/genetics/immunology Monocytes/cytology Proto-Oncogene Proteins c-fos/genetics/immunology RNA, Messenger/genetics/immunology Dewey 540 Dewey 616.07 39 Département de biochimie 144 137 Département de pathologie et immunologie 21F false 221 Groupe Reith Walter (pathologie et immunologie) 282 572 Groupe Santiago-Raber Marie-Laure (pathologie et immunologie) 915 Blood 117 17 9e2ecbb1-f24f-47b0-bccf-b442d580bd2f Article (Published version) Restricted Dunand-Sauthier W Reith 2011.pdf 1222644 application/pdf restrictedAccess Faculté des sciences / Section de chimie et biochimie / Département de biochimie Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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Silencing of c-Fos expression by microRNA-155 is critical for dendritic cell maturation and function	Blood			2011	658	0

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Capponi, Léonardo Piero